Overview

Also Known As: S1Pr1, S1pr1, S1P₁^loxP

These S1pr1^loxP/loxP mice possess loxP sites flanking exon 2. They may have applications in studies related to adaptive immune responses and vascular development.

Donating Investigator

Richard L Proia, National Institutes of Health

Genetic overview

Genetic Background	Generation
	N7+N1F15 (2019-03-01 00:00:00)

S1pr1^tm2.1Rlp

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	S1pr1	sphingosine-1-phosphate receptor 1

View Genetics

Research Applications

Cardiovascular Research
Developmental Biology Research
Research Tools
Immunology, Inflammation and Autoimmunity Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

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Details

Detailed Description

These S1pr1^loxP/loxP mice possess loxP sites flanking exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. S1PR1 is a G protein-coupled receptor for lysophospholipid sphingosine-1-phosphate (S1P) and is highly expressed in endothelial cells. S1PR1 is essential for vascular maturation during embryonic development and is also involved in cell survival, migration, adhesion, and proliferation. This receptor plays a role in the regulation of innate and adaptive immune responses by controlling lymphocyte egress from the thymus, spleen, bone marrow, and lymph nodes. It has also been implicated in the regulation of vascular function. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Tek-cre)1Ywa/J (Stock No. 008863)
Mice expressing Cre recombinase in vascular endothelial cells during embryogenesis and adulthood, the resulting offspring die by E14.5 due to muscle defects including heart, vascular smooth muscle, and skeletal muscle defects.

Development

A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Offspring, heterozygous for this S1pr1^loxP allele, were bred with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J transgenic mice (Stock No. 003724) to delete the neo cassette. Resulting mice contained multiple gene rearrangments; intact floxed-exon 2, intact floxed-neo cassette, or excision of both exon 2 and the neo cassette. Progeny, containing only the floxed-exon 2, were crossed to remove the Cre-expressing transgene. The donating investigator reported that these mice were bred to C57BL/6NCrl mice for at least 7 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6J genetic background.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Allende ML; Yamashita T; Proia RL. 2003. G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation. Blood 102(10):3665-7PubMed: 12869509 MGI: J:86450

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Genetics

S1pr1^tm2.1Rlp

Allele Symbol: S1pr1^tm2.1Rlp

Allele Name	targeted mutation 2.1, Richard L Proia
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	S1pr1^tm2.1Rlp; targeted mutation 2.1, Richard L Proia
Gene Symbol and Name	S1pr1, sphingosine-1-phosphate receptor 1
Gene Synonym(s)	S1P; EDG-1; endothelial differentiation spingolipid G-protein-coupled receptor 1; ECGF1; CD363; EDG1; Edg1; S1P1; CHEDG1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; expressed sequence AI849002; AI849002; D1S3362; Edg1
Strain of Origin	129S6/SvEvTac
Chromosome	3
Molecular Note	A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2. Cre-mediated recombination removed the neo cassette and left exon 2 floxed.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cardiovascular Research
- Vascular Defects
- Heart Abnormalities
Developmental Biology Research
- Internal/Organ Defects
  - heart: vasculature
  - vasculature
Research Tools
- Cardiovascular Research
  - endothelial cell marker for neovascularization
- Cancer Research
  - endothelial cell marker for neovascularization
- Cre-lox System
  - loxP-flanked Sequences
Immunology, Inflammation and Autoimmunity Research
- Lymphoid Tissue Defects
  - Lymphocyte Homing
Cancer Research
- Genes Regulating Growth and Proliferation

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: S1pr1^tm2.1Rlp/S1pr1⁺
involves: 129S6/SvEvTac

mammalian phenotype

no phenotypic analysis

References

Allende ML; Yamashita T; Proia RL. 2003. G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation. Blood 102(10):3665-7PubMed: 12869509 MGI: J:86450

Additional - S1pr1^tm2.1Rlp related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: S1pr1^tm2Rlp
- End Point Analysis: Crb1^rd8End Point
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the S1P₁^loxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #019141 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX18 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for S1pr1<tm2.1Rlp>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: S1Pr1, S1pr1, S1P1loxP

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

S1pr1tm2.1Rlp

Allele Symbol: S1pr1tm2.1Rlp

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: S1pr1tm2.1Rlp/S1pr1+involves: 129S6/SvEvTac

mammalian phenotype

References

Additional - S1pr1tm2.1Rlp related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: S1Pr1, S1pr1, S1P₁^loxP

S1pr1^tm2.1Rlp

Allele Symbol: S1pr1^tm2.1Rlp

Genotype: S1pr1^tm2.1Rlp/S1pr1⁺
involves: 129S6/SvEvTac

Additional - S1pr1^tm2.1Rlp related