A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Offspring, heterozygous for this S1pr1^loxP allele, were bred with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J transgenic mice (Stock No. 003724) to delete the neo cassette. Resulting mice contained multiple gene rearrangments; intact floxed-exon 2, intact floxed-neo cassette, or excision of both exon 2 and the neo cassette. Progeny, containing only the floxed-exon 2, were crossed to remove the Cre-expressing transgene. The donating investigator reported that these mice were bred to C57BL/6NCrl mice for at least 7 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6J genetic background.

Approximate Controls

• 000664 C57BL/6J
• 005304 C57BL/6NJ

Additional Information

• Considerations for Choosing Controls

• Allende ML; Yamashita T; Proia RL. 2003. G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation. Blood 102(10):3665-7PubMed: 12869509MGI: J:86450