BALB/c-Gt(ROSA)26Sor^{tm10(Lmp1)Rsky}/J

Stock number: 019120
Research areas: Research Tools, Virology Research

Description

These LMP1^flSTOP mice allow inducible expression of EBV latent membrane protein 1 and have applications in studies of Epstein-Barr virus-induced immune surveillance and lymphomagenesis, and EBV-driven lymphoproliferative disorders.

Detailed description

These mice contain EBV latent membrane protein 1, Lmp1, coding sequence inserted into the Gt(ROSA)26Sor locus. LMP-1 (Epstein-Barr virus latent membrane protein 1) is a six-span transmembrane protein that is a functional, but dysregulated, mimic of CD40 signaling to B cells. Expression of the Lmp1 gene is blocked by a loxP-flanked STOP fragment placed between the Lmp1 sequence and the Gt(ROSA)26Sor promoter. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre expressing tissue resulting in expression of Lmp1. Mice that are homozygous for the targeted mutation are viable and fertile.

For example, when bred to mice carrying Cd19^tm1(cre)Cgn (Stock No. 006785), Cre recombinase expression results in expression of LMP1 in B cells causing lymphoproliferation and lymphomagenesis.

Development

A targeting vector containing a splice acceptor, a floxed Neo^r-STOP cassette and the EBV latent membrane protein 1 (Lmp1) coding sequence (EBV B95-8 strain derived) was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into BALB/cJ derived Balb/C-I embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to BALB/c mice. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cJ (Stock No. 000651) at least once to establish the colony.

Allele

Symbol: Gt(ROSA)26Sor^{tm10(Lmp1)Rsky}
Name: targeted mutation 10, Klaus Rajewsky
MGI accession ID: MGI:5314029
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence
Synonyms: LMP1^flSTOP
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: BALB/cJ
Expressed genes: EBVS1,Epstein Barr virus integration site 1,human
Molecular note: The construct containing (5' to 3') a splice acceptor, floxed neo stop cassette and EBV B95-8 strain Lmp1 coding sequence was inserted into the locus.