These LMP1flSTOP mice allow inducible expression of EBV latent membrane protein 1 and have applications in studies of Epstein-Barr virus-induced immune surveillance and lymphomagenesis, and EBV-driven lymphoproliferative disorders.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These mice contain EBV latent membrane protein 1, Lmp1, coding sequence inserted into the Gt(ROSA)26Sor locus. LMP-1 (Epstein-Barr virus latent membrane protein 1) is a six-span transmembrane protein that is a functional, but dysregulated, mimic of CD40 signaling to B cells. Expression of the Lmp1 gene is blocked by a loxP-flanked STOP fragment placed between the Lmp1 sequence and the Gt(ROSA)26Sor promoter. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre expressing tissue resulting in expression of Lmp1. Mice that are homozygous for the targeted mutation are viable and fertile.
For example, when bred to mice carrying Cd19tm1(cre)Cgn (Stock No. 006785), Cre recombinase expression results in expression of LMP1 in B cells causing lymphoproliferation and lymphomagenesis.
A targeting vector containing a splice acceptor, a floxed Neor-STOP cassette and the EBV latent membrane protein 1 (Lmp1) coding sequence (EBV B95-8 strain derived) was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into BALB/cJ derived Balb/C-I embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to BALB/c mice.
Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cJ (Stock No. 000651) at least once to establish the colony.
Expressed Gene | EBVS1, Epstein Barr virus integration site 1, human |
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Site of Expression |
Allele Name | targeted mutation 10, Klaus Rajewsky |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) |
Allele Synonym(s) | LMP1flSTOP |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | EBVS1, Epstein Barr virus integration site 1, human |
Strain of Origin | BALB/cJ |
Chromosome | 6 |
Molecular Note | The construct containing (5' to 3') a splice acceptor, floxed neo stop cassette and EBV B95-8 strain Lmp1 coding sequence was inserted into the locus. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the BALB/c-Gt(ROSA)26Sortm10(Lmp1)Rsky/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019120 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm10(Lmp1)Rsky> |
Frozen Mouse Embryo | BALB/c-Gt(ROSA)26Sor<tm10(Lmp1)Rsky>/J | $2595.00 |
Frozen Mouse Embryo | BALB/c-Gt(ROSA)26Sor<tm10(Lmp1)Rsky>/J | $2595.00 |
Frozen Mouse Embryo | BALB/c-Gt(ROSA)26Sor<tm10(Lmp1)Rsky>/J | $3373.50 |
Frozen Mouse Embryo | BALB/c-Gt(ROSA)26Sor<tm10(Lmp1)Rsky>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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