B6;129P2-Tmeff2^tm1Fawa/J

Stock number: 019119
Research areas: Developmental Biology Research, Research Tools

Description

Tmeff2^PLAP knock-in/out mice express alkaline phosphatase in central, peripheral and enteric neurons, and have applications in visualization of neuronal innervation.

Detailed description

Tmeff2, a transmembrane protein with EGF-like and two follistatin-like domains 2, encodes a tumor suppressor for prostate and other cancers. It serves a growth factor for neurons and other cells. These Tmeff2^PLAP mice express the human alkaline phosphatase, placental gene (ALPP) from a disrupted endogenous Tmeff2 locus. Homozygotes are smaller in size than controls and die around weaning age (at about 3 weeks of age). Heterozygotes are viable and fertile. Alkaline phosphatase activity is detected in central, peripheral and enteric neurons, and as early as embryonic day 12.5 in the brain. No gene product (mRNA) is detected by in situ hybridization analysis of dorsal root ganglion. Homozygotes have reduced white adipose tissue when compared to wildtype controls.

Development

A targeting vector containing a hPLAP, human alkaline phosphatase, placental gene (ALPP), and an ACN cassette was used to disrupt exon 1. The ACN cassette, containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter, is flanked by loxP sites. Cre-mediated recombination during spermatogenesis removed the cassette leaving one loxP site. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for a total of 2 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tmeff2^tm1Fawa
Name: targeted mutation 1, Fan Wang
MGI accession ID: MGI:5438565
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Tmeff2
Marker name: transmembrane protein with EGF-like and two follistatin-like domains 2
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Expressed genes: ALPP,alkaline phosphatase, placental,human
Molecular note: A construct including a cDNA encoding human ALPP and a floxed neo cassette was inserted after the initiation codon replacing the rest of exon 1. In situ hybridization confirmed the absence of mRNA expression in homozygous embryos.