Dlk1flox mice may be useful for studying Dlk1 function in the development and regeneration of both myogenic progenitor cells and mature skeletal muscles.
Jennifer Schmidt, University of Illinois at Chicago
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Dlk1 | delta like non-canonical Notch ligand 1 |
These Dlk1flox mice possess loxP sites flanking the last two exons of the delta-like 1 homolog (Dlk1) gene. Dlk1 is an epidermal growth factor (EGF)-like protein expressed from the paternal allele in skeletal muscle and cartilage. Expression is also seen in the developing pituitary gland, pancreas, lung, adrenal gland, and placenta. Since Dlk1 is paternally inherited, the donating investigator maintains these mice as heterozygotes, with the mutated allele always originating from the male parent. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the last two exons deleted in cre-expressing tissues. Because Dlk1 is imprinted and only expressed from the paternal allele, breeding Dlk1flox males with cre-expressing females is required to generate deleted offspring with the knockout phenotype.
For example, when males are crossed to
B6.129S4-Myf5tm3(cre)Sor/J (Stock No. 007893) females expressing Cre recombinase in muscle progenitor cells, resulting mice exhibit defective muscle development, elevated inflammatory responses, and delayed muscle regeneration.
A targeting vector was designed by Dr. Jennifer Schmidt (University of Illinois at Chicago) to surround the last 2 exons of the delta-like 1 homolog (Dlk1) gene with loxP sites. A neomycin resistance (neo) cassette followed by a single loxP site was inserted downstream of the final exon. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting mice were bred to C57BL/6J mice and their offspring were bred to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock No. 003724) transgenic mice. Offspring contained multiple gene rearrangments; intact floxed-exons, intact floxed-neo cassette, or excision of both floxed-exons and the neo cassette. Male Dlk1flox offspring, containing only the floxed-exons, were bred with C57BL/6J females. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Jennifer V Schmidt |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dlk1flox |
Gene Symbol and Name | Dlk1, delta like non-canonical Notch ligand 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 12 |
Molecular Note | A loxP site and a neo cassette flanked by two loxP sites were inserted to flank the last two exons (5 and 6). The neo cassette was removed in vivo by Cre-mediated recombination. |
Mutations Made By | Jennifer Schmidt, University of Illinois at Chicago |
For routine maintenance of the Dlk1flox colony, homozygotes may be bred together.
Because Dlk1 is imprinted and only expressed from the paternal allele, breeding Dlk1flox males with cre-expressing females is required to generate deleted offspring with the knockout phenotype.
When using the Dlk1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #019074 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Dlk1<tm1.1Jvs> |
Frozen Mouse Embryo | STOCK Dlk1<tm1.1Jvs>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dlk1<tm1.1Jvs>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dlk1<tm1.1Jvs>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Dlk1<tm1.1Jvs>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.