The Cck-IRES-Cre allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, cre expression is directed by the endogenous Cck promoter/enhancer elements. When Cck-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cck-expressing cells in the offspring. Cck expression from the Cck-IRES-Cre allele has not been evaluated. Additional phenotype information described below.
For characterization information, see images at the Allen Institute for Brain Science website (Cck-IRES-Cre images).
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Ccktm1.1(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.
The parental line, Cck-IRES-Cre knockin mice on a C57BL/6;129 genetic background, are available and described as Stock No. 012706. It should be noted that the phenotype of these C57BL/6NJ-congenic Cck-IRES-Cre knockin mice (Stock No. 019021) could vary from that of the parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
