GluA1 knockout mice exhibit behavioral, social, and cognitive deficits. They may have applications in studies related to behavioral, social and cognitive abnormalities, hippocampal synaptic transmission/plasticity, and nociception, as well as neuropsychiatric disorders such as schizophrenia and depression/mania.
Rolf Sprengel, Max Planck Institute for Medical Res
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gria1 | glutamate receptor, ionotropic, AMPA1 (alpha 1) |
The GluA1 knockout allele (also called GluA1-, GluR-A-, or A1-lox) has a deletion of the sequences necessary in formation of the glutamate receptor transmembrane domain ion channel pore (exon 11). No GluA1 protein expression is detected from the targeted allele. Homozygous mice are viable and fertile, with behavioral, social, and cognitive deficits. Specifically, homozygotes have profound impairment of several different spatial working memory tasks and selective impairment on short-term recognition memory tasks. Homozygotes show no alterations on spatial reference memory tasks or long-term memory tasks. GluA1-/- mice also have novelty- and stress-induced hyperactivity, and increased anxiety-related responses. Hippocampal CA1 pyramidal neurons from homozygous mice have strongly reduced extrasynaptic AMPA currents and do not show the dendritic distance-dependent scaling of excitatory synaptic strengths seen in wildtype neurons.
A targeting vector was designed to insert a loxP site upstream of exon 11, and a loxP-flanked neo cassette (from ploxpneo3) downstream of exon 11 of the glutamate receptor, ionotropic, AMPA1 [alpha 1] gene (Gria1; GluR-A, GluA1). This loxP::exon11::loxP::neo::loxP construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells with the GluR-Aneo allele (also called 3loxP allele) were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the GluR-A- genotype (both exon 11 and neo selection cassette removed; leaving a single loxP site remaining) were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6 mice to establish the mutant colony. The donating investigator reports that GluR-A- mice were subsequently backcrossed to C57BL/6NCrl mice for ten generations prior to sending to The Jackson Laboratory Repository in 2012. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Allele Name | targeted mutation 1, Rolf Sprengel |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GluA1-; GluR1-; GluR-A-; GluRA- |
Gene Symbol and Name | Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1) |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
General Note | Phenotypic Similarity to Human Syndrome: Schizoaffective Disorder (J:167264). |
Molecular Note | A single loxP site was inserted into intron 10 and a loxP flanked neomycin selection cassette was inserted into exon 11. The loxP-flanked sequences were deleted in ES cells by transient Cre expression prior to the production of chimeric mice. The final heritable allele contains a single loxP site replacing exon 11 and flanking intronic sequences. Immunohistochemistry experiments confirmed that no detectable protein is expressed from this allele. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.129-Gria1tm1Rsp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019011 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wildtype for Gria1<tm1Rsp> |
Frozen Mouse Embryo | B6N.129-Gria1<tm1Rsp>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129-Gria1<tm1Rsp>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129-Gria1<tm1Rsp>/J | $3373.50 |
Frozen Mouse Embryo | B6N.129-Gria1<tm1Rsp>/J | $3373.50 |
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