Exon 3 is deleted from these Smarcc2 mutant mice, causing a protein frame shift. Homozygotes die within 3 days of birth.
Gerald R Crabtree, Stanford University Medical Center
A neomycin cassette flanked by loxP sites was introduced to intron 2 and a loxP site was inserted in intron 3 using TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. Resultant mice were crossed with a Cre strain on a C57BL/6 background to excise the neomycin cassette and exon 3. This deletion creates a protein frame shift after amino acid 77. This strain has been backcrossed to C57BL/6 for 3 or 4 generations by the donating lab.
|Allele Name||targeted mutation 1.1, Gerald R Crabtree|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Smarcc2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A neomycin cassette flanked by loxP sites was introduced to intron 2 and a loxP site was inserted in intron 3. Cre-mediated recombination removed the neo cassette and exon 3. This deletion creates a protein frame shift after amino acid 77.|
Heterozygotes are viable and fertile, but homozygotes die within 3 days of birth.
When using the B6;129S-Smarcc2tm1.1Grc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019010 in your Materials and Methods section.