A hygro cassette replaces the entire coding region of the CD27 antigen (Cd27; Tnfrsf7) gene, abolishing gene expression. Homozygotes are viable and fertile. CD27 is a tumor necrosis factor (TNF) receptor family member that in mice is found on hematopoietic stem cells in bone marrow, thymocytes, naive and activated peripheral T cells, subsets of natural killer (NK) cells and activated B cells. CD27 expression can be lost from effector T cells and is absent from IL-17-producing gamma delta T cells. CD27 is not expressed outside of the lymphoid lineage. Upon binding of its ligand, CD70, on activated immune cells, CD27 drives clonal expansion of primed T cells in lymphoid organs and survival of effector T cells in non-lymphoid organs. CD27-deficient mice have a more narrow responder TCR repertoire to antigenic challenge. CD27 also supports effector function of T cells and NK cells and memory formation and function of T cells. CD27-deficient mice have normal conventional T cell and B cell development, but a mildly impaired regulatory T cell development. They have mild to moderate impairments in primary and particularly memory T cell responses to viruses, dependent on the virus type or strain. In the mouse, B cell function is not overtly affected by CD27-deficiency. In the mouse, activated immune cells bearing CD70 can exert feed-back control on hematopoiesis via CD27 on hematopoietic precursors.

These Cd27 knockout mice have impaired T cell responses to viruses as well as delayed memory cell responses. They may have applications in studies related to
immune cell development and peripheral expansion.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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