PKCeta-/- mice may be useful for studying T cell homeostasis and activation.
Nicholas Gascoigne, The Scripps Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Prkch | protein kinase C, eta |
Exon 2 of the protein kinase C, eta (Prkch) gene was removed, abolishing gene function. These PKCeta-/- homozygotes are viable and fertile. Prkch-encoded PKCeta is a serine/threonine kinase abundant in T cells which is recruited during positive selection of thymocytes. T-cells from these PKCeta-/- mice showed poor proliferation in response to antigen stimulation and exhibited defective homeostatic proliferation. These mice also have a higher ratio of CD4+ to CD8+ T cells compared to that of wild-type mice.
A targeting vector was designed to insert a loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 and a second loxP site downstream of exon 2 of the protein kinase C, eta (Prkch) gene. The construct was electroporated into C57BL/6-derived Brunce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) to delete the neo cassette, then the progeny were bred with B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock No. 003724) transgenic mice to delete exon 2. progeny were crossed to remove the cre- and Flp-expressing transgenes. These mice were then backcrossed to C57BL/6J mice for at least 4 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.2, Nicholas Gascoigne |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Prkch, protein kinase C, eta |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 12 |
Molecular Note | A targeting vector was designed to insert a loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 and a second loxP site downstream of exon 2. Flp-mediated recombination removed the neo cassette while cre-mediated recombination remvoed exon 2. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.Cg-Prkchtm1.2Gasc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018988 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Prkch<tm1.2Gasc> |
Frozen Mouse Embryo | B6.Cg-Prkch<tm1.2Gasc>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Prkch<tm1.2Gasc>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Prkch<tm1.2Gasc>/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Prkch<tm1.2Gasc>/J | $3373.50 |
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