These Dio1 knockout mice may be useful in applications involving hyperthyroidism, iodine deficiency, and metabolism regulation.
Valerie Anne Galton, Dartmouth Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Dio1 | deiodinase, iodothyronine, type I |
A neo cassette replaces exon 2 of the deiodinase, iodothyronine, type I (Dio1) gene, including the TGA selenocysteine codon, abolishing gene function in these D1KO mice. D1 is a selenoenzyme expressed in the liver, kidney, thyroid, and pituitary where it generates triiodothyronine (T3) thyroid hormone from thyroxine (T4) for export to the plasma. D1 is also a scavenger enzyme, playing a role in iodide conservation in peripheral tissues and the thyroid. Homozygotes are viable and fertile. D1KO mice exhibit increased fecal excretion of iodothyronines and increased levels of T4 and reverse (r)T3 in serum.
A targeting vector was designed to replace exon 2 of the deiodinase, iodothyronine, type I (Dio1) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129-dervived DS2A embryonic stem (ES) cells and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to C57BL/6 females and subsequently a colony of D1KO mice were established. D1KO mice were then mated to D2KO mice deficient of the deiodinase, iodothyronine, type 2 (Dio2) gene. These double mutant mice were backcrossed for at least 10 generations to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation and the D1KO and D2KO alleles were separated. D2KO mice are maintained a Stock No. 018985.
Allele Name | targeted mutation 1, Valerie Anne Galton |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | D1KO |
Gene Symbol and Name | Dio1, deiodinase, iodothyronine, type I |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 4 |
General Note | ES cell line = DS2A |
Molecular Note | Exon two, containing the TGA selenocysteine codon, was replaced with the neo cassette. Protein activity was not detected in mutant liver, kidney, thyroid or pituitary tissues. |
Mutations Made By | Valerie Anne Galton, Dartmouth Medical School |
When maintaining a live colony, homozygous mice may be bred together.
When using the D1KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #018984 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dio1<tm1Vag> |
Frozen Mouse Embryo | B6.129-Dio1<tm1Vag>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Dio1<tm1Vag>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Dio1<tm1Vag>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Dio1<tm1Vag>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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