Overview

Also Known As:Sst-IRES-Cre, SOM-IRES-Cre

Sst-IRES-Cre knock-in mice express Cre recombinase in somatostatin-expressing neurons. These mice may be useful in studying dendritic inhibitory interneurons such as Martinotti cells and Oriens-Lacunosum-Moleculare cells.
While Sst-IRES-Cre was designed as a 3' knock-in allele, additional characterization indicates it has significantly diminished endogenous Sst expression - see details below. As such, researchers should consider using heterozygous AVP-IRES2-Cre-D mice and wildtype littermate controls in all their studies.
Of note, the same Sst-IRES-Cre knock-in allele is also available on a C57BL/6J genetic background as Stock No. 028864.

Donating Investigator

IMR Colony, The Jackson Laboratory

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	N4pN3F7 (2019-12-31 00:00:00)

Sst^{tm2.1(cre)Zjh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Knockdown)	Sst	somatostatin

View Genetics

Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

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Details

Detailed Description

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. The phenotype summarized below is for the parental line: Sst-IRES-Cre knock-in mice on a mostly C57BL/6;129S4 genetic background (Stock No. 013044). It should be noted that the phenotype of these C57BL/6NJ-congenic Sst-IRES-Cre knock-in mice (Stock No. 018973) could vary from that of the parental line from which it was derived. The phenotype below describes the parental line (Stock No. 013044).

The Sst-IRES-Cre knock-in allele (or SOM-IRES-Cre) has an internal ribosome entry site and Cre recombinase in the 3' UTR of the somatostatin locus (Sst). As such, the endogenous Sst promoter/enhancer elements direct cre expression to somatostatin-expressing neurons. While Sst-IRES-Cre was designed as a 3' knock-in allele, additional characterization indicates it has significantly diminished endogenous Sst expression - see details below. As such, researchers should consider using heterozygous AVP-IRES2-Cre-D mice and wildtype littermate controls in all their studies.
When Sst-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Sst-expressing cells in the offspring.

In 2010, the donating investigator of Stock No. 013044 reported Cre recombinase activity is specific and efficient; largely recapitulating the endogenous somatostatin expression pattern with efficient recombination. They reported Cre recombinase activity is observed in somatostatin positive neurons (including dendritic inhibitory interneurons such as Martinotti cells and Oriens-Lacunosum-Moleculare (O-LM) cells). The donating investigator did not examine cre expression in tissues other than brain. Sst expression from the Sst-IRES-Cre allele was not evaluated. They also reported that homozygous mice were viable, fertile and normal in size, with no gross physical abnormalities or behavioral abnormalities.

Although Sst-IRES-Cre was designed as a 3' knock-in allele, additional characterization indicates it has significantly diminished endogenous Sst expression. Specifically, in 2016, unpublished research using Stock No. 013044 reported the Sst-IRES-Cre allele had diminished Sst RNA expression, and homozygous mice had abnormal locomotor activity (reduced in males during circadian cycle active phase, increased in females by the end of circadian cycle active phase). Heterozygous mice had partial recovery of Sst expression and normal behavioral responses. Furthermore, the findings of another group confirmed Sst-IRES-Cre imparts an allele dosage-dependent knock-down of endogenous Sst expression [Viollet et al. 2017 Front Endocrinol (Lausanne) 8:131 (PMID:28674519)]. Researchers researchers should consider using heterozygous Sst-IRES-Cre mice and wildtype littermate controls in their studies.

For characterization information of the Sst-IRES-Cre knock-in allele, see images at the Allen Institute for Brain Science website (Sst-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Sst^{tm2.1(cre)Zjh}). This same information would also be found searching the MGI Recombinase Activity database.

Development

Mice harboring the Sst-IRES-Cre (or SOM-IRES-Cre) knock-in allele on a mixed genetic background (mostly C57BL/6) were sent to The Jackson Laboratory Repository in 2010 (as Stock No. 013044).

In 2012, to be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018973). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 98% of the SNP markers as C57BL/6N allele-type.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in somatostatin positive neurons (including dendritic inhibitory interneurons such as Martinotti cells and Oriens-Lacunosum-Moleculare cells).

Control Suggestions

Wild-type from the colony
005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Genetics

Sst^{tm2.1(cre)Zjh}

Allele Symbol: Sst^{tm2.1(cre)Zjh}

Allele Name	targeted mutation 2.1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing, Knockdown)
Allele Synonym(s)	SOM-iCre; SOM-IRES-Cre; SSTCre; Sst-iCre; Sst-IRES-Cre
Gene Symbol and Name	Sst, somatostatin
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in somatostatin positive neurons (including dendritic inhibitory interneurons such as Martinotti cells and Oriens-Lacunosum-Moleculare cells).
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	16
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the somatostatin locus (Sst). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette.This mutation results in a dosage-dependent knock-down of endogenous Sst expression J:279254 .
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Sst^{tm2.1(cre)Zjh}/Sst^{tm2.1(cre)Zjh}
involves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities

References

Additional References

Garcia-Junco-Clemente P; Ikrar T; Tring E; Xu X; Ringach DL; Trachtenberg JT. 2017. An inhibitory pull-push circuit in frontal cortex. Nat Neurosci 20(3):389-392PubMed: 28114295 MGI: J:271925
Groisman AI; Yang SM; Schinder AF. 2020. Differential Coupling of Adult-Born Granule Cells to Parvalbumin and Somatostatin Interneurons. Cell Rep 30(1):202-214.e4PubMed: 31914387 MGI: J:287623
Yaeger CE; Ringach DL; Trachtenberg JT. 2019. Neuromodulatory control of localized dendritic spiking in critical period cortex. Nature 567(7746):100-104PubMed: 30787434 MGI: J:274346

Additional - Sst^{tm2.1(cre)Zjh} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Sst Alternate1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
Researchers should consider using heterozygous Sst-IRES-Cre mice and wildtype littermate controls in their studies - please see strain description for more details.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the Sst-IRES-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018973 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wild-type for Sst<tm2.1(cre)Zjh>

Related Products and Services

Frozen Mouse Embryo	B6N.Cg-Sst<tm2.1(cre)Zjh>/J	$2595.00
Frozen Mouse Embryo	B6N.Cg-Sst<tm2.1(cre)Zjh>/J	$2595.00
Frozen Mouse Embryo	B6N.Cg-Sst<tm2.1(cre)Zjh>/J	$3373.50
Frozen Mouse Embryo	B6N.Cg-Sst<tm2.1(cre)Zjh>/J	$3373.50

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:Sst-IRES-Cre, SOM-IRES-Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Ssttm2.1(cre)Zjh

Allele Symbol: Ssttm2.1(cre)Zjh

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Ssttm2.1(cre)Zjh/Ssttm2.1(cre)Zjhinvolves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

References

Additional References

Additional - Ssttm2.1(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Sst^{tm2.1(cre)Zjh}

Allele Symbol: Sst^{tm2.1(cre)Zjh}

Genotype: Sst^{tm2.1(cre)Zjh}/Sst^{tm2.1(cre)Zjh}
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Sst^{tm2.1(cre)Zjh} related