These CaMKIIalpha-cre T29-1 transgenic mice have the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter driving Cre recombinase expression in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 146/148 markers (99%) as C57BL/6N allele-type. Both of the segregating markers are on chromosome 5 (~18.2-41.1 Mbp).
It should be noted that the Cre expression pattern and/or phenotype of these CaMKIIalpha-cre T29-1 transgenic mice (Stock No. 018966) could vary from that of the parental line originally described on a different genetic background (Stock No. 005359). We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
Mice harboring the CaMKIIalpha-cre T29-1 transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2005 (as Stock No. 005359). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018966). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 146/148 markers (99%) as C57BL/6N allele-type. Both of the segregating markers are on chromosome 5 (~18.2-41.1 Mbp).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | forebrain; pyramidal cell layer |
Allele Name | transgene insertion T29-1, Susumu Tonegawa |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Camk2a-cre; CaMKIIalpha-cre; T29-1 |
Gene Symbol and Name | Tg(Camk2a-cre)T29-1Stl, transgene insertion T29-1, Susumu Tonegawa |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | forebrain; pyramidal cell layer |
Strain of Origin | (C57BL x BALB/c)F1 |
Chromosome | UN |
Molecular Note | This transgene directs the expression of Cre recombinase under the control of the alpha subunit of the calcium/calmodulin-dependent protein kinase II promoter. Cre recombinase activity was detected in the CA1 region of the hippocampus. |
Mutations Made By | Dr. Susumu Tonegawa, Massachusetts Institute of Technology |
Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6NJ inbred mice.
For Cre-lox experiments and to avoid germline deletion/global recombination of floxed alleles, researchers may consider breeding Tg(Camk2a-cre)T29-1Stl females with floxed males. See Detailed Description for more details.
When using the B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018966 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Camk2a-cre)T29-1Stl |
Frozen Mouse Embryo | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J Frozen Embryo | $3373.50 |
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