B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J

Stock number: 018966
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

These CaMKIIalpha-cre T29-1 transgenic mice have the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter driving Cre recombinase expression in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus.

Detailed description

To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 146/148 markers (99%) as C57BL/6N allele-type. Both of the segregating markers are on chromosome 5 (~18.2-41.1 Mbp).

It should be noted that the Cre expression pattern and/or phenotype of these CaMKIIalpha-cre T29-1 transgenic mice (Stock No. 018966) could vary from that of the parental line originally described on a different genetic background (Stock No. 005359). We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

Development

Mice harboring the CaMKIIalpha-cre T29-1 transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2005 (as Stock No. 005359). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018966). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 146/148 markers (99%) as C57BL/6N allele-type. Both of the segregating markers are on chromosome 5 (~18.2-41.1 Mbp).

Allele

Symbol: Tg(Camk2a-cre)T29-1Stl
Name: transgene insertion T29-1, Susumu Tonegawa
MGI accession ID: MGI:2177650
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Camk2a-cre; CaMKIIalpha-cre; T29-1
Marker symbol: Tg(Camk2a-cre)T29-1Stl
Marker name: transgene insertion T29-1, Susumu Tonegawa
Marker synonyms: CaMKIIalpha-cre
Chromosome: UN
Strain of origin: (C57BL x BALB/c)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: forebrain; pyramidal cell layer
Molecular note: This transgene directs the expression of Cre recombinase under the control of the alpha subunit of the calcium/calmodulin-dependent protein kinase II promoter. Cre recombinase activity was detected in the CA1 region of the hippocampus.