The LysMcre knock-in/knockout allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements. These LysMcre mice may be useful for Cre-lox studies of the myeloid cell lineage (monocytes, mature macrophages and granulocytes) and the innate immune response.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Lyz2 | lysozyme 2 |
To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.
The parental line, C57BL/6-congenic LysMcre knockin/knockout mice, are available and described as Stock No. 004781. It should be noted that the phenotype of these C57BL/6NJ-congenic LysMcre knockin/knockout mice (Stock No. 018956) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
Mice harboring the LysMcre knockin/knockout allele on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2003 (as Stock No. 004781).
In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018956). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | myeloid cells - including monocytes, mature macrophages, and granulocytes |
Allele Name | targeted mutation 1, Irmgard Forster |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | (LyzM)-Cre; LCre; LC; LysCre; Lys-cre; LysM Cre; lysM/cre; LysMCre; LysMCre; LysM-Cre; Lystm1(cre)Ifo; Lyz2Cre; Lyz2-Cre; LyzCre; Lyz-cre |
Gene Symbol and Name | Lyz2, lysozyme 2 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | myeloid cells - including monocytes, mature macrophages, and granulocytes |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 10 |
Molecular Note | An NLS-cre gene was inserted into the endogenous ATG start site of the gene. An frt-flanked neomycin cassette was removed in ES cells via Flp-mediated recombination prior to production of chimeric mice. |
Mutations Made By | Irmgard Foerster, University of Duesseldorf |
Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the LysMcre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018956 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wild-type for Lyz2<tm1(cre)Ifo>/ |
Frozen Mouse Embryo | B6N.129P2(B6)-Lyz2<tm1(cre)Ifo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129P2(B6)-Lyz2<tm1(cre)Ifo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129P2(B6)-Lyz2<tm1(cre)Ifo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129P2(B6)-Lyz2<tm1(cre)Ifo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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