B6N.129P2(B6)-Lyz2^tm1(cre)Ifo/J

Stock number: 018956
Product name: LysMcre
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

The LysMcre knock-in/knockout allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements. These LysMcre mice may be useful for Cre-lox studies of the myeloid cell lineage (monocytes, mature macrophages and granulocytes) and the innate immune response.

Detailed description

To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.

The parental line, C57BL/6-congenic LysMcre knockin/knockout mice, are available and described as Stock No. 004781. It should be noted that the phenotype of these C57BL/6NJ-congenic LysMcre knockin/knockout mice (Stock No. 018956) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

Development

Mice harboring the LysMcre knockin/knockout allele on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2003 (as Stock No. 004781).

In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018956). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.

Allele

Symbol: Lyz2^tm1(cre)Ifo
Name: targeted mutation 1, Irmgard Forster
MGI accession ID: MGI:1934631
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Lyz2
Marker name: lysozyme 2
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: myeloid cells - including monocytes, mature macrophages, and granulocytes
Molecular note: An NLS-cre gene was inserted into the endogenous ATG start site of the gene. An frt-flanked neomycin cassette was removed in ES cells via Flp-mediated recombination prior to production of chimeric mice.