This strain is useful for permanent labeling of a rare and transient subpopulation of mouse embryonic stem (ES) cells that lack the typical inner cell mass pluripotency markers found in two-cell (2C) embryos but can contribute to the development of both embryonic and extraembryonic tissues.
Samuel L Pfaff, The Salk Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
A rare and transient subpopulation of mouse embryonic stem (ES) cells can express high levels of transcripts that are found in two-cell (2C) embryos. These cells lack the typical inner cell mass pluripotency markers Oct4 (Pou5f1), Sox2, and Nanog but can contribute to the development of both embryonic and extraembryonic tissues. It was found that these ES cells cycle in and out of the pluripotent 2C-like state.
The most highly activated sequence repeat in two-cell (2C) embryos is the MuERV-L (murine endogenous retrovirus with leucine tRNA primer) family of retroviruses and their corresponding long terminal repeat (LTR) promoters (Mt2_mm).
In order to indelibly mark these cells and follow their fate, a transgenic mouse line incorporating MuERV-L regulatory elements driving expression of a tamoxifen-inducible Cre recombinase was created (2C::ERT2-Cre-ERT2).
When crossed with Gt(ROSA)26Sor floxed stop lacZ reporter strain (see Stock No. 007905), progeny may be treated with 4-hydroxytamoxifen (4HT) to detect nuclear Cre expression in MuERV-L Gag+ cells. ES cells derived from these mice that are cultured in 4HT show a steady increase in the percentage of reporter-positive cells.
The 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L, also called MERVL and Erv4) and the primer binding site drive expression of tamoxifen-inducible Cre recombinase (2C::ERT2-Cre-ERT2). Two flanking ERT2 segments were inserted to make Cre activation less leaky. The transgenic vector was injected into CB6 (BALB/c and C57BL/6) embryos and the resultant strain was subsequently maintained on the same mixed genetic background by the donating laboratory. This is founder line 657.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | ES cells derived from 4-hydroxytamoxifen treated mice show a steady increase in the percentage of reporter-positive cells |
Allele Name | transgene insertion 657, Samuel L Pfaff |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | 2C::ERT2-Cre-ERT2; Tg(MuERV-L-ERT2-Cre-ERT2)657Slp |
Gene Symbol and Name | Tg(Erv4-cre/ERT2*)657Slp, transgene insertion 657, Samuel L Pfaff |
Gene Synonym(s) | |
Promoter | Erv4, endogenous retroviral sequence 4 (with leucine tRNA primer), mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | ES cells derived from 4-hydroxytamoxifen treated mice show a steady increase in the percentage of reporter-positive cells |
Strain of Origin | BALB/c and C57BL/6 |
Chromosome | UN |
Molecular Note | The 5' LTR of murine endogenous retrovirus with leucine tRNA primer (MuERV-L; MERVL; Erv4) and the primer binding site drive expression of tamoxifen-inducible Cre recombinase (2C::ERT2-Cre-ERT2). Two flanking ERT2 segments were inserted to make Cre activation less leaky. The transgenic vector was injected into CB6 (BALB/c and C57BL/6) embryos and the resultant strain was subsequently maintained on the same mixed genetic background by the donating laboratory. This is founder line 657. |
Hemizygotes are viable, fertile, and have no overt phenotype. It is not yet known whether homozygotes are viable and fertile.
When using the B6;C-Tg(Erv4-cre/ERT2*)657Slp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018937 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Erv4-cre/ERT2*)657Slp |
Frozen Mouse Embryo | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J | $2595.00 |
Frozen Mouse Embryo | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J | $2595.00 |
Frozen Mouse Embryo | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J | $3373.50 |
Frozen Mouse Embryo | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J | $3373.50 |
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