129S(B6)-Vps26a^tm1Cos/J

Stock number: 018925
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research

Description

These KO mice may be useful in studies examining the effect of retromer deficiency on Aβ peptide accumulations, neurodegeneration and membrane trafficking.

Detailed description

Exon 2 of the vacuolar protein sorting 26 homolog A (Vps26a) gene was replaced with a neo cassette, abolishing gene function. Vps26a gene product, Hβ58, is expressed in the hippocampal formation as part of a large multimeric complex called the retromer complex. This complex is involved in protein trafficking from endosomes to the trans-Golgi network. Homozygotes display delayed development beginning at E7.5 and embryonic arrest at about E9.5. Heterozygous mice are viable and fertile, and develop hippocampal-dependent memory and synaptic dysfunction associated with elevations concentrations of Aβ peptide.

Development

A targeting vector was designed to replace exon 2 of the vacuolar protein sorting 26 homolog A (Vps26a) gene with a neomycin(neo) resistance cassette and a polyadenylation sequence. The construct was electroporated into 129S/SvEv-Gpi1^c-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were backcrossed to 129/SvEvTac mice for at least 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.

Allele

Symbol: Vps26a^tm1Cos
Name: targeted mutation 1, Frank Costantini
MGI accession ID: MGI:3610386
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Hbeta58^-
Marker symbol: Vps26a
Marker name: VPS26 retromer complex component A
Marker synonyms: H beta 58; HB58; Hbeta58; PEP8A; VPS26
Chromosome: 10
Strain of origin: 129S/SvEv-Gpi1^c
Molecular note: A targeting vector was designed to replace exon 2 of the vacuolar protein sorting 26 homolog A gene with a neomycin(neo) resistance cassette and a polyadenylation sequence. (direct communication)