These Notch3d1 mutation is associated with abnormal arterial wall morphology. This mutant mouse strain may be useful in studies of the Notch pathway during development, vascular development and angiogenesis.
Thomas Gridley, Maine Medical Center Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Notch3 | notch 3 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot analysis of brain, lung and heart tissue or Western blot analysis of lung and brain tissue from homozygotes. Homozygotes exhibit disorganized artery wall morphology, and thin vascular smooth muscle cell coat and processes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a PGK-neo cassette was used to delete 2.5 kbp of sequence encoding the EGF repeats 8 through 12. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric males were bred to C57BL/6J females for germline transmission. The Notch3d1 colony was then backcrossed to C57BL/6J inbred mice (Stock No. 000664) for three generations prior to sending to The Jackson Laboratory Repository in 2010 as Stock No. 010547. Upon arrival, mice were bred to C57BL/6J inbred mice to establish our live colony. In January 2014, the colony was bred once to B6129SF1/J mice (Stock No. 101043). Mice on a mixed C57BL/6;129S1 genetic background are Stock No. 018910.
Allele Name | targeted mutation 1, Tom Gridley |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Notch3d1 |
Gene Symbol and Name | Notch3, notch 3 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 17 |
Molecular Note | A 2.5 kb genomic fragment containing sequence encoding EGF repeats 8 through 12 was replaced with a PGK-neo cassette inserted by homologous recombination. The absence of transcript and protein was indicated by Northern and Western blot analysis of homozygous mutant mice. |
Mutations Made By | Thomas Gridley, Maine Medical Center Research Institute |
When maintaining the live colony, heterozygous mice may be bred to wildtype (noncarrier) mice from the colony. Alternatively, homozygous mice may be bred together.
When using the Notch3d1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #018910 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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