On a low potassium diet, Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) homozygous mice exhibit decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. This strain may be useful for studying potassium and sodium homeostasis.
Gary E Shull, University of Cincinnati
Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) encodes the alpha-subunit of a P-type family of ion transport ATPases found in the colon, kidneys and prostate. cHKA mediates K+ absorption in the colonic epithelium. Homozygous mice are viable and fertile. On a low potassium diet, mice decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. On a low sodium diet, mice exhibit an impaired absorption of sodium and potassium, increased serum aldosterone and increased potassium excretion. cHKA-deficient mice also are associated with loss of acidification of luminal prostate fluids and prostate tumors. This strain may be useful for studying potassium and sodium homeostasis.
A targeting vector containing the neomycin resistance gene was used to disrupt exon 20, which contains the transmembrane domains critical for ion transport. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to Black Swiss mice. Mice from the colony were backcrossed to BALB/c for at least 10 generations. Upon arrival, mice were bred to BALB/cJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Gary E Shull|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||cHKA -|
|Gene Symbol and Name||Atp12a, ATPase, H+/K+ transporting, nongastric, alpha polypeptide|
|Strain of Origin||Not Specified|
|Molecular Note||A nenomycin selection cassette was inserted into exon 20. Northern blot and RT-PCR analysis on RNA derived from colon of homozygous mice demonstrated that aberrantly spliced transcripts were produced from this allele, but none of these produced a functional message.|
|Mutations Made By|| |
Gary Shull, University of Cincinnati
While maintaining a live colony, these mice are bred as homozygotes.
When using the C.129X1(Cg)-Atp12atm1Ges/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36713 in your Materials and Methods section.