On a low potassium diet, Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) homozygous mice exhibit decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. This strain may be useful for studying potassium and sodium homeostasis.
Gary E Shull, University of Cincinnati
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Atp12a | ATPase, H+/K+ transporting, nongastric, alpha polypeptide |
Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) encodes the alpha-subunit of a P-type family of ion transport ATPases found in the colon, kidneys and prostate. cHKA mediates K+ absorption in the colonic epithelium. Homozygous mice are viable and fertile. On a low potassium diet, mice decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. On a low sodium diet, mice exhibit an impaired absorption of sodium and potassium, increased serum aldosterone and increased potassium excretion. cHKA-deficient mice also are associated with loss of acidification of luminal prostate fluids and prostate tumors. This strain may be useful for studying potassium and sodium homeostasis.
A targeting vector containing the neomycin resistance gene was used to disrupt exon 20, which contains the transmembrane domains critical for ion transport. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to Black Swiss mice. Mice from the colony were backcrossed to BALB/c for at least 10 generations. Upon arrival, mice were bred to BALB/cJ for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Gary E Shull |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | cHKA - |
Gene Symbol and Name | Atp12a, ATPase, H+/K+ transporting, nongastric, alpha polypeptide |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 14 |
Molecular Note | A nenomycin selection cassette was inserted into exon 20. Northern blot and RT-PCR analysis on RNA derived from colon of homozygous mice demonstrated that aberrantly spliced transcripts were produced from this allele, but none of these produced a functional message. |
Mutations Made By | Gary Shull, University of Cincinnati |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C.129X1(Cg)-Atp12atm1Ges/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36713 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.