Overview

On a low potassium diet, Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) homozygous mice exhibit decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. This strain may be useful for studying potassium and sodium homeostasis.

Donating Investigator

Gary E Shull, University of Cincinnati

Genetic overview

Genetic Background	Generation

Atp12a^tm1Ges

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Atp12a	ATPase, H+/K+ transporting, nongastric, alpha polypeptide

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Research Applications

Metabolism Research
Internal/Organ Research

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Details

Detailed Description

Atp12a or cHKA (H+/K+ transporting, nongastric ATPase, alpha polypeptide) encodes the alpha-subunit of a P-type family of ion transport ATPases found in the colon, kidneys and prostate. cHKA mediates K⁺ absorption in the colonic epithelium. Homozygous mice are viable and fertile. On a low potassium diet, mice decreased potassium levels in the colon, plasma and muscle (in excess of wild-type) and weight loss. On a low sodium diet, mice exhibit an impaired absorption of sodium and potassium, increased serum aldosterone and increased potassium excretion. cHKA-deficient mice also are associated with loss of acidification of luminal prostate fluids and prostate tumors. This strain may be useful for studying potassium and sodium homeostasis.

Development

A targeting vector containing the neomycin resistance gene was used to disrupt exon 20, which contains the transmembrane domains critical for ion transport. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to Black Swiss mice. Mice from the colony were backcrossed to BALB/c for at least 10 generations. Upon arrival, mice were bred to BALB/cJ for at least 1 generation to establish the colony.

Control Suggestions

Wild-type from the colony
000651 BALB/cJ

Additional Information

Considerations for Choosing Controls

Selected References

Meneton P; Schultheis PJ; Greeb J; Nieman ML; Liu LH; Clarke LL; Duffy JJ; Doetschman T; Lorenz JN; Shull GE. 1998. Increased sensitivity to K+ deprivation in colonic H,K-ATPase-deficient mice. J Clin Invest 101(3):536-42PubMed: 9449685 MGI: J:45707

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Genetics

Atp12a^tm1Ges

Allele Symbol: Atp12a^tm1Ges

Allele Name	targeted mutation 1, Gary E Shull
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	cHKA -
Gene Symbol and Name	Atp12a, ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Gene Synonym(s)
Strain of Origin	Not Specified
Chromosome	14
Molecular Note	A nenomycin selection cassette was inserted into exon 20. Northern blot and RT-PCR analysis on RNA derived from colon of homozygous mice demonstrated that aberrantly spliced transcripts were produced from this allele, but none of these produced a functional message.
Mutations Made By	Gary Shull, University of Cincinnati

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Metabolism Research
- Enzyme Deficiency
Internal/Organ Research
- Gastrointestinal Defects

Mammalian Phenotype Terms by Genotype

References

Meneton P; Schultheis PJ; Greeb J; Nieman ML; Liu LH; Clarke LL; Duffy JJ; Doetschman T; Lorenz JN; Shull GE. 1998. Increased sensitivity to K+ deprivation in colonic H,K-ATPase-deficient mice. J Clin Invest 101(3):536-42PubMed: 9449685 MGI: J:45707

Additional - Atp12a^tm1Ges related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Atp12a
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the C.129X1(Cg)-Atp12a^tm1Ges/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36713 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Atp12atm1Ges

Allele Symbol: Atp12atm1Ges

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Atp12atm1Ges related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Atp12a^tm1Ges

Allele Symbol: Atp12a^tm1Ges

Additional - Atp12a^tm1Ges related