B6;129-Fyb^tm1Gak/J

Stock number: 018862
Product name: ADAP KO
Research areas: Cardiovascular Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These Fyb knockout mice display defective T cell receptor-mediated adhesion and aggregation. They may have applications in studies related to leukocyte recruitment.

Detailed description

A neo cassette replaces exon 1 of the FYN binding protein (Fyb) gene, abolishing gene expression. FYB, also known as ADAP (adhesion and degranulation promoting adaptor protein), is expressed in various hematopoietic cell types and is required for slow leukocyte rolling and leukocyte recruitment to areas of inflammation. The number of T cells in these mice is decreased and they display defective T cell receptor (TCR)-mediated adhesion and aggregation. These mice also have decreased numbers of platelets and leukocytes. Homozygotes are viable and fertile.

Development

A targeting vector was designed to replace exon 1 of the FYN binding protein (Fyb) gene with a neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. The donating investigator reported that these mice were backcrossed 10 generations to C57BL/6 mice (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Two of the 27 markers throughout the genome are segregating, suggesting an incomplete backcross.

Allele

Symbol: Fyb^tm1Gak
Name: targeted mutation 1, Gary A Koretzky
MGI accession ID: MGI:3663737
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fyb
Marker name: FYN binding protein
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 1 was replaced with a neomycin resistance cassette.