These Fyb knockout mice display defective T cell receptor-mediated adhesion and aggregation. They may have applications in studies related to leukocyte recruitment.
Gary Koretzky, University of Pennsylvania
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Fyb | FYN binding protein |
A neo cassette replaces exon 1 of the FYN binding protein (Fyb) gene, abolishing gene expression. FYB, also known as ADAP (adhesion and degranulation promoting adaptor protein), is expressed in various hematopoietic cell types and is required for slow leukocyte rolling and leukocyte recruitment to areas of inflammation. The number of T cells in these mice is decreased and they display defective T cell receptor (TCR)-mediated adhesion and aggregation. These mice also have decreased numbers of platelets and leukocytes. Homozygotes are viable and fertile.
A targeting vector was designed to replace exon 1 of the FYN binding protein (Fyb) gene with a neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. The donating investigator reported that these mice were backcrossed 10 generations to C57BL/6 mice (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Two of the 27 markers throughout the genome are segregating, suggesting an incomplete backcross.
Allele Name | targeted mutation 1, Gary A Koretzky |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ADAP-; Fyb- |
Gene Symbol and Name | Fyb, FYN binding protein |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | Exon 1 was replaced with a neomycin resistance cassette. |
Mutations Made By | Gary Koretzky, University of Pennsylvania |
When maintaining a live colony, homozygous mice may be bred together.
When using the ADAP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #018862 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fyb<tm1Gak>/ |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.