These Lcp2 knockout mice exhibit a complete loss of T cells and γδ-TCR+ lymphocytes, but maintain a normal number of B cells, macrophages, neutrophils, mast cells and NK cells. They may be useful for studies related to T cell development.
Gary Koretzky, University of Pennsylvania
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lcp2 | lymphocyte cytosolic protein 2 |
SLP-76 is an immune cell adaptor expressed in T lymphocytes, macrophages, mast cells, platelets, neutrophils, and natural killer (NK) cells. It has been shown to play a role in lymphatic vascular development, T cell receptor (TCR) signaling, as well as signaling through receptors that use tyrosine base activation motifs in platelet, mast cells, neutrophils and NK cells. In these lymphocyte cytosolic protein 2 (Lcp2) knockout mice, a neomycin resistance cassette in reverse orientation disrupts the gene and abolishes Lcp2 expression. These mice have small lymph nodes and enlarged spleens when compared to controls. They exhibit a complete loss of T cells and γδ-TCR+ lymphocytes, but maintain a normal number of B cells, macrophages, neutrophils, mast cells and NK cells. Some homozygotes may be viable, but are born at a frequency lower than typical Mendelian ratios would predict. Substantial perinatal lethality is observed.
A targeting vector was designed to replace 360 bp including exon 1 and the translational start site of the lymphocyte cytosolic protein 2 (Lcp2) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. The donating investigator reports that these mice were backcrossed for at least 10 generations to C57BL/6J mice (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Gary A Koretzky |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | SLP-76- |
Gene Symbol and Name | Lcp2, lymphocyte cytosolic protein 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | Approximately 360 bp of the gene, including part of exon 1 and the translation initiation site, was replaced with a neo cassette via homologous recombination. RT-PCR analysis of homozygous mutant animals verified the absence of gene expression. Western blot and immunohistochemistry of spleen from homozygous mutant animals did not detect protein product. |
Mutations Made By | Gary Koretzky, University of Pennsylvania |
When maintaining a live colony, heterozygous mice may be bred together. The donating investigator reports that homozygotes are viable but born at a frequency lower than Mendelian, with substantial perinatal lethality.
When using the SLP-76- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018860 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Lcp2<tm1Gak> |
Frozen Mouse Embryo | B6.129-Lcp2<tm1Gak>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Lcp2<tm1Gak>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Lcp2<tm1Gak>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Lcp2<tm1Gak>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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