B6;129S1-Kcnn4^tm1Jemn/J

Stock number: 018826
Product name: KCa3.1-
Research areas: Cell Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

These mice may be useful for studying the role of calcium-activated intermediate potassium channels on fluid transport, sickle disease, and inflammatory bowel diseases.

Detailed description

In this strain, a neo cassette replaces exon 1 of the potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4) gene, abolishing gene function. Kcnn4 encodes the calcium-activated intermediate potassium (IK) channel, KCa3.1, in red blood cells, T lymphocytes, and cells in organs involved in salt and fluid transport, including the colon, lung, and salivary glands. Kcnn4 null mice do not express IK channels in their parotid acinar cells, their red blood cells are not K⁺ permeable, and T lymphocytes and erythrocytes exhibit impaired volume regulation. These mice are protected from developing Crohn's disease and ulcerative colitis in adoptive transfer studies. Homozygotes are viable and fertile.

Development

A targeting vector was designed to replace exon 1 of the potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females to generate a colony of KCa3.1^-/- mice. These mice were bred to C57BL/6J mice for at least 2 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Kcnn4^tm1Jemn
Name: targeted mutation 1, James E Melvin
MGI accession ID: MGI:3521668
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Kcnn4
Marker name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Chromosome: 7
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 1 was deleted upon incorporation of a PGK-neo cassette into the locus. Northern blot showed a lack of transcript in mutant CD4+ T lymphocytes.