These mice may be useful for studying the role of calcium-activated intermediate potassium channels on fluid transport, sickle disease, and inflammatory bowel diseases.
James Melvin, National Institutes of Health
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Kcnn4 | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 |
In this strain, a neo cassette replaces exon 1 of the potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4) gene, abolishing gene function. Kcnn4 encodes the calcium-activated intermediate potassium (IK) channel, KCa3.1, in red blood cells, T lymphocytes, and cells in organs involved in salt and fluid transport, including the colon, lung, and salivary glands. Kcnn4 null mice do not express IK channels in their parotid acinar cells, their red blood cells are not K+ permeable, and T lymphocytes and erythrocytes exhibit impaired volume regulation. These mice are protected from developing Crohn's disease and ulcerative colitis in adoptive transfer studies. Homozygotes are viable and fertile.
A targeting vector was designed to replace exon 1 of the potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females to generate a colony of KCa3.1-/- mice. These mice were bred to C57BL/6J mice for at least 2 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, James E Melvin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IK1-; KCa3.1; KCa3.1-; Kcnn4- |
Gene Symbol and Name | Kcnn4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 7 |
Molecular Note | Exon 1 was deleted upon incorporation of a PGK-neo cassette into the locus. Northern blot showed a lack of transcript in mutant CD4+ T lymphocytes. |
Mutations Made By | James Melvin, National Institutes of Health |
When maintaining a live colony, homozygotes may be bred together.
When using the KCa3.1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018826 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Kcnn4<tm1Jemn> |
Frozen Mouse Embryo | B6;129S1-Kcnn4<tm1Jemn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Kcnn4<tm1Jemn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Kcnn4<tm1Jemn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S1-Kcnn4<tm1Jemn>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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