These Trp63-cre/cerulean transgenic mice express cre recombinase and the cerulean variant of green fluorescent protein (GFP) driven by transformation related protein 63 (Trp63) promoter/enhancer elements. They may be useful for generating conditional mutations for studying craniofacial development.
Steve Murray, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice hemizygous for the Trp63-cre/cerulean transgene express cre recombinase and the cerulean variant of green fluorescent protein (GFP) under direction of transformation related protein 63 (Trp63) promoter/enhancer elements. Uniform cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Patchy cre activity is observed in nasal epithelium. Uniform cre activity is also seen in submandibular gland epithelium. When these mice are bred with mice containing a loxP-flanked sequence, cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring.
View cre expression characterization.
In the laboratory of Dr. Steve Murray at the Jackson Laboratory, a transgene was designed to contain (from 5' to 3') the entire mouse transformation related protein 63 (Trp63) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 4 was bred to C57BL/6J females (Stock No. 000664).
Upon arrival in The Jackson Laboratory Repository, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Uniform cre activity is also seen in submandibular gland epithelium. Patchy cre activity is observed in nasal epithelium. |
Allele Name | transgene insertion 4, Genetic Resource Science Repository |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Trp63,-cre,-Cerulean)4Grsr, transgene insertion 4, Genetic Resource Science Repository |
Gene Synonym(s) | |
Promoter | Trp63, transformation related protein 63, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Uniform cre activity is also seen in submandibular gland epithelium. Patchy cre activity is observed in nasal epithelium. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A transgene was designed to contain (from 5' to 3') the entire mouse transformation related protein 63 (Trp63) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistancecassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 4 was bred to establish line 4. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the Trp63-cre/cerulean mouse strain in a publication, please cite the originating article(s) and include JAX stock #018792 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Trp63,-cre,-Cerulean)4Grsr |
Frozen Mouse Embryo | C57BL/6J-Tg(Trp63 -cre -Cerulean)4Grsr/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Trp63 -cre -Cerulean)4Grsr/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Trp63 -cre -Cerulean)4Grsr/GrsrJ | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Trp63 -cre -Cerulean)4Grsr/GrsrJ | $3373.50 |
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