| Allele Name | transgene insertion 4, Genetic Resource Science Repository |
| Allele Type | Transgenic (Recombinase-expressing) |
| Allele Synonym(s) | |
| Gene Symbol and Name | Tg(Trp63,-cre,-Cerulean)4Grsr, transgene insertion 4, Genetic Resource Science Repository |
| Gene Synonym(s) | |
| Promoter | Trp63, transformation related protein 63, mouse, laboratory |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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| Site of Expression | Cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Uniform cre activity is also seen in submandibular gland epithelium. Patchy cre activity is observed in nasal epithelium. |
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| Strain of Origin | C57BL/6J |
| Chromosome | UN |
| Molecular Note | A transgene was designed to contain (from 5' to 3') the entire mouse transformation related protein 63 (Trp63) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistancecassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 4 was bred to establish line 4. |
