C57BL/6J-Tg(Trp63,-cre,-Cerulean)4Grsr/GrsrJ

Stock number: 018792
Product name: Trp63-cre/cerulean
Research areas: Developmental Biology Research, Research Tools

Description

These Trp63-cre/cerulean transgenic mice express cre recombinase and the cerulean variant of green fluorescent protein (GFP) driven by transformation related protein 63 (Trp63) promoter/enhancer elements. They may be useful for generating conditional mutations for studying craniofacial development.

Detailed description

Mice hemizygous for the Trp63-cre/cerulean transgene express cre recombinase and the cerulean variant of green fluorescent protein (GFP) under direction of transformation related protein 63 (Trp63) promoter/enhancer elements. Uniform cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Patchy cre activity is observed in nasal epithelium. Uniform cre activity is also seen in submandibular gland epithelium. When these mice are bred with mice containing a loxP-flanked sequence, cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring.

View cre expression characterization.

Development

In the laboratory of Dr. Steve Murray at the Jackson Laboratory, a transgene was designed to contain (from 5' to 3') the entire mouse transformation related protein 63 (Trp63) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 4 was bred to C57BL/6J females (Stock No. 000664). Upon arrival in The Jackson Laboratory Repository, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.

Allele

Symbol: Tg(Trp63,-cre,-Cerulean)4Grsr
Name: transgene insertion 4, Genetic Resource Science Repository
MGI accession ID: MGI:5485018
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Trp63,-cre,-Cerulean)4Grsr
Marker name: transgene insertion 4, Genetic Resource Science Repository
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: cre,cre recombinase,bacteriophage P1; GFP,Green Fluorescent Protein,
Site of expression: Cre activity has been detected at E14.5 throughout both oral and cranial epithelium. Oral epithelial expression includes anterior and posterior secondary palatal shelf epithelium, oral and nasal epithelial triangles, medial edge epithelium, primary palate epithelium, and tooth buds. Uniform cre activity is also seen in submandibular gland epithelium. Patchy cre activity is observed in nasal epithelium.
Molecular note: A transgene was designed to contain (from 5' to 3') the entire mouse transformation related protein 63 (Trp63) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 4 was bred to establish line 4.