B6.129S-Pknox1^tm1.1Xzh/J

Stock number: 018780
Product name: Prep1^flox
Research areas: Developmental Biology Research, Research Tools

Description

These Prep1^flox mutant mice possess loxP sites flanking exon 8 of the Pbx/knotted 1 homeobox (Pknox1) gene. They may have applications in studies examining the role of Pax6 on lens and pancreas development, and tumor suppression.

Detailed description

These Prep1^flox mutant mice possess loxP sites flanking exon 8 of the Pbx/knotted 1 homeobox (Pknox1) gene. PREP1 is an upstream enhancer of PAX6 signaling required for lens and pancreas development. PREP1-deficiency is implicated in the development of some spontaneous cancers. Mice that are homozygous for this allele are viable, fertile, and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in cre-expressing tissues. PREP1 deficiency in the lens and pancreas do not result in developmental defects.

Development

A targeting vector was designed to insert a loxP site upstream of exon 8 and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 8 of the Pbx/knotted 1 homeobox (Pknox1) gene. The construct was electroporated into 129S6/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring, heterozygous for this Prep1^flox allele, were bred with B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ transgenic mice (Stock No. 009086) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6J mice for at least 6 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Pknox1^tm1.1Xzh
Name: targeted mutation 1.1, Xin Zhang
MGI accession ID: MGI:5317868
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Prep1^flox
Marker symbol: Pknox1
Marker name: Pbx/knotted 1 homeobox
Marker synonyms: D17Wsu76e; pkonx1c; PREP1
Chromosome: 17
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP site was inserted upstream of exon 8 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 8 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 8 floxed.