These mice are useful when studying sulfotransferase conversion of cholesterol and other hormone substrates.
Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sult2b1 | sulfotransferase family, cytosolic, 2B, member 1 |
In this strain, exons 4-8 of the sulfotransferase 2B1 (Sult2b1) gene were removed, abolishing gene expression. SULT2B1 is a sulfotransferase enzyme that transfer an SO3- group from the cofactor 3'-phosphoadenosine 5'-phosphate to the 3-hydroxyl position of cholesterol and other substrates such as some hormones and neurotransmitters. SULT2B1 is highly expressed in the skin of the mouse and at lower levels in the epididymis. Male and female homozygous Sult2b1 knockout mice are viable and fertile. They lack cholesterol sulfate in the dermis but otherwise appear to have normal lipid metabolism.
A targeting vector was designed to replace exons 4-8 of the sulfotransferase 2B1 (Sult2b1) gene with an ACN cassette. The ACN cassette, containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter, is flanked by loxP sites. Cre-mediated recombination during spermatogenesis removed the ACN cassette leaving one loxP site. The construct was electroporated into 129S6/SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to 129/SvJ females. The donating investigator reports that these mice were maintained on an isogenic 129/SvJ background (see SNP note below) and embryos were shipped to The Jackson Laboratory. Recovered mice were bred together to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome suggested a C57BL/6 genetic contribution from an unknown source.
Allele Name | targeted mutatioin 1, David Russell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Sult2b1, sulfotransferase family, cytosolic, 2B, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | A targeting vector was designed to replace the seven coding exons the sulfotransferase 2B1 (Sult2b1) gene with a cassette containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter. This cassette was flanked by loxP sites. Cre-mediated recombination during spermatogenesis removed the inserted cassette leaving one loxP site. |
Mutations Made By | Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas |
When maintaining a live colony, homozygous mice may be bred together.
When using the Sult2b1b KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #018773 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Sult2b1<tm1Rus> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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