B6;129-Sult2b1^tm1Rus/J

Stock number: 018773
Product name: Sult2b1b KO
Research areas: Metabolism Research

Description

These mice are useful when studying sulfotransferase conversion of cholesterol and other hormone substrates.

Detailed description

In this strain, exons 4-8 of the sulfotransferase 2B1 (Sult2b1) gene were removed, abolishing gene expression. SULT2B1 is a sulfotransferase enzyme that transfer an SO^3- group from the cofactor 3'-phosphoadenosine 5'-phosphate to the 3-hydroxyl position of cholesterol and other substrates such as some hormones and neurotransmitters. SULT2B1 is highly expressed in the skin of the mouse and at lower levels in the epididymis. Male and female homozygous Sult2b1 knockout mice are viable and fertile. They lack cholesterol sulfate in the dermis but otherwise appear to have normal lipid metabolism.

Development

A targeting vector was designed to replace exons 4-8 of the sulfotransferase 2B1 (Sult2b1) gene with an ACN cassette. The ACN cassette, containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter, is flanked by loxP sites. Cre-mediated recombination during spermatogenesis removed the ACN cassette leaving one loxP site. The construct was electroporated into 129S6/SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to 129/SvJ females. The donating investigator reports that these mice were maintained on an isogenic 129/SvJ background (see SNP note below) and embryos were shipped to The Jackson Laboratory. Recovered mice were bred together to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome suggested a C57BL/6 genetic contribution from an unknown source.

Allele

Symbol: Sult2b1^tm1Rus
Name: targeted mutation 1, David Russell
MGI accession ID: MGI:5432568
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sult2b1
Marker name: sulfotransferase family, cytosolic, 2B, member 1
Marker synonyms: ARCI14; Gm5897; HSST2; ST2B1; SULT2B
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to replace the seven coding exons the sulfotransferase 2B1 (Sult2b1) gene with a cassette containing the neomycin resistance gene and Cre recombinase gene under the control of angiotensin-converting enzyme promoter. This cassette was flanked by loxP sites. Cre-mediated recombination during spermatogenesis removed the inserted cassette leaving one loxP site.