Cyp8b1 KO mice are useful when studying the role of cholic acid in lipid metabolism.
Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cyp8b1 | cytochrome P450, family 8, subfamily b, polypeptide 1 |
In this strain, the cytochrome P450, family 8, subfamily b, polypeptide 1 (Cyp8b1) gene is replaced with a β-galactosidase (lacZ) sequence and a neomycin resistance (neo) cassette in reverse orientation, abolishing gene expression. Cyp8b1 is expressed in the liver and is required for the synthesis of the bile acid, cholic acid. Cholic acid is required for the absorption of dietary long-chain fatty acids and cholesterol in the intestine, and in the liver suppresses endogenous bile acid synthesis. Cyp8b1 KO mice fail to produce cholic acid, have increased bile acid synthesis, and reduce the absorption of dietary lipids. Homozygotes are viable and fertile.
A targeting vector was designed to replace the entire coding region of the cytochrome P450, family 8, subfamily b, polypeptide 1 (Cyp8b1) gene with a β-galactosidase (lacZ) sequence and a neomycin resistance (neo) cassette in reverse orientation. The construct was electroporated into 129S7/SvEvBrd-Hprt+-derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were maintained on a mixed C57BL/6J;129 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David Russell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cyp8b1- |
Gene Symbol and Name | Cyp8b1, cytochrome P450, family 8, subfamily b, polypeptide 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 9 |
Molecular Note | The coding region was replaced with beta-galactosidase coding sequence and a neomycin resistance gene in reverse orientation. Transcript was not detected in livers of mutant mice. |
Mutations Made By | Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S7-Cyp8b1tm1Rus/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018771 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cyp8b1<tm1Rus> |
Frozen Mouse Embryo | B6;129S7-Cyp8b1<tm1Rus>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Cyp8b1<tm1Rus>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Cyp8b1<tm1Rus>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Cyp8b1<tm1Rus>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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