B6;D-Tg(Wnt11-TagRFP/cre/ERT2)28Amc/J

Stock number: 018683
Research areas: Developmental Biology Research, Research Tools

Description

These Wnt11-myrTagRFP-IRES-CE transgenic mice utilize a tamoxifen inducible Cre-mediated recombination system and may be useful in studies of genitourinary system development.

Detailed description

These Wnt11-myrTagRFP-IRES-CE mutant mice have a tamoxifen inducible Cre-mediated recombination system and express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Wnt11, wingless-related MMTV integration site 11, promoter. RFP fluorescence is detected by fluorescent microscope in the renal ureteric tips and collecting duct system and the lung at of 15.5 dpc age wholemount embryos. Direct (native) RFP fluorescence co-localizes with Cre activity in the developing collecting ducts, and immunohistochemical labeling of TagRFP co-localizes with Cre activity, in the ureteric tips. Tamoxifen inducible Cre recombinase activity is detected in ureteric buds and tree, the developing collecting ducts, male and female reproductive system and the lung. Mice that are hemizygous for the knock in allele are viable and fertile. The expected coat color is black or brown.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

A targeting construct containing a myristoylated Tag-RFPT variant of Red Fluorescent Protein, cre/ERT2 and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Wnt11 gene in the RP23-122D14 BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting modified Wnt11-myrTagRFP-IRES-CE BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 28 was subsequently established. The mice were then crossed to C57BL/6 for 3 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Wnt11-TagRFP/cre/ERT2)28Amc
Name: transgene insertion 28, Andrew P McMahon
MGI accession ID: MGI:5435691
Generation method: Transgenic
Attributes: Recombinase-expressing; Reporter; Inducible
Marker symbol: Tg(Wnt11-TagRFP/cre/ERT2)28Amc
Marker name: transgene insertion 28, Andrew P McMahon
Chromosome: UN
Strain of origin: (C57BL/6 x DBA)F1
Expressed genes: RFP,Red Fluorescent Protein,coral; cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: RFP fluorescence and tamoxifen inducible cre activity is detected in the renal ureteric tips, collecting duct system, and the lung at E15.5.
Molecular note: A targeting construct containing a myristoylated Tag-RFPT variant of Red Fluorescent Protein, cre/ERT2, and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Wnt11 gene in the RP23-122D14 BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting modified Wnt11-myrTagRFP-IRES-CE BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 28 was subsequently established.