| Allele Name | transgene insertion 30, Andrew P McMahon |
| Allele Type | Transgenic (Recombinase-expressing, Reporter, Inducible) |
| Allele Synonym(s) | Meox1-nuc-TagRFP-T-IRES-CE |
| Gene Symbol and Name | Tg(Meox1-TagRFP/cre/ERT2)30Amc, transgene insertion 30, Andrew P McMahon |
| Gene Synonym(s) | |
| Promoter | Meox1, mesenchyme homeobox 1, mouse, laboratory |
|---|
| Expressed Gene | RFP, Red Fluorescent Protein, coral |
|---|
| Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
|---|
| Site of Expression | Tamoxifen inducible Cre recombinase activity is detected in Six2 (sine oculis-related homeobox 2 homolog (Drosophila) ) positive cap mesenchyme cells in the developing nephron at 15.5dpc. |
|---|
| Strain of Origin | (C57BL/6 x DBA)F1 |
| Chromosome | UN |
| Molecular Note | A targeting construct containing a nuclear-localized Tag-RFPT variant of Red Fluorescent Protein, cre/ERT2 and FRT site flanked Kanamycin/neomycin selection cassette was inserted into theconsensus start ATG codon of the mouse Meox1 gene in the RP23-5O22 BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting modified nucTagRFPT-ires-CE BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 30 was subsequently established. |
| Mutations Made By | Andrew McMahon, University of Southern California |
