129P3/J-Chr 7^C57BL/6ByJ/MonMmjax

Stock number: 018677
Product name: 129P3/J-Chr 7
Research areas: Research Tools

Description

This strain represents one of a panel of chromosome substitution (or consomic) strains. Each strain carries a chromosome from the C57BL/6ByJ strain introgressed into a 129P3/J background. These strains represent a tool for dissection of quantitative trait loci. In particular, these two strains exhibit differences in body composition, alcohol consumption and taste responsiveness.

Detailed description

This strain represents one of a panel of chromosome substitution (or consomic) strains. Each strain carries a chromosome from the C57BL/6ByJ strain introgressed into a 129P3/J background. These strains represent a tool for dissection of quantitative trait loci (QTL). In particular, these two strains exhibit differences in taste response, ingestive behavior, alcohol consumption, body size, and adiposity. Genome scans of crosses between these strains have detected QTLs for these phenotypes that cluster on Chr 1, 2, 7 and 9 (Adip2; Adip3, Adip5, Ap7q, Bwq5, Bwq6, Nattq1, Nattq2, Sucq).

Development

Dr. Alexander Bachmanov of the Monell Chemical Senses Center developed this reciprocal consomic set for Chromosomes 1, 2, 7, and 9 by replacing individual chromosomes from the C57BL/6ByJ donor strain into the 129P3/J host strain using a marker-assisted series of backcrosses. The set was donated to the MMRRC at The Jackson Laboratory in 2012.

In 2013, a 154 SNP (single nucleotide polymorphism) panel analysis covering all 19 chromosomes and the X chromosome was performed at The Jackson Laboratory. All markers on Chr 7 are fixed for the C57BL/6 allele type. The remaining markers are fixed for 129P3/J with the exception of 3 markers on Chr 8 and 1 marker on Chr 10 that tested as heterozygous for C57BL/6 and 129P3/J.

Allele

Symbol: Ap7q^C57BL/6ByJ
Name: C57BL/6ByJ
MGI accession ID: MGI:2668895
Generation method: QTL
Marker symbol: Ap7q
Marker name: alcohol preference 7 QTL
Chromosome: 7
Strain of origin: C57BL/6ByJ
Molecular note: This allele confers increased intake of 10% ethanol solution compared to 129P3/J.

Allele

MGI accession ID: MGI:2149043
Marker symbol: Adip3
Marker name: adiposity 3
Chromosome: 7