Overview

Also Known As:Foxp3EGFP

EGFP is expressed from the forkhead box P3 (Foxp3) locus in these mice. Foxp3 expression is not disrupted. Foxp3⁺ T_reg cells can be identified in these mice by EGFP expression.

Donating Investigator

Bernard Malissen, Centre d'immunologie de Marseille Luminy

Genetic overview

Genetic Background	Generation

Foxp3^tm1Mal

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Foxp3	forkhead box P3

View Genetics

Research Applications

Research Tools

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

An IRES-EGFP cassette was placed in exon 11 in the 3' untranslated region of the X-linked forkhead box P3 (Foxp3) gene, without disrupting expression of the endogenous gene in this mutant mouse line. FOXP3 is a transcription factor necessary for the development and function of regulatory T (T reg) cells, which are required for suppression of self-reactive T cells and prevention of some autoimmune diseases. In these mice, EGFP expression is evident in FOXP3+ Treg cells. Homozygous Foxp3GFP mice are viable and fertile.

This new model from Dr Bernard Malissen of Centre d'Immunologie de Marseille-Luminy, France, targets the IRES-GFP cassette to exon 11, downstream of the endogenous Foxp3 STOP codon with no defects in FOXP3 or regulatory T cell function observed. This strain serves as an alternative to B6.Cg-Foxp3^tm2Tch/J (Stock No. 006772) which targets the IRES-GFP cassette in intron 9 of the coding region.

Development

The targeting vector was designed to insert (from 5' to 3') an internal ribosomal entry site (IRES) fused to an enhanced green fluorescent protein (EGFP) and a self-excising loxP-flanked ACN cassette into exon 11 of the X-linked forkhead box P3 (Foxp3) gene. The ACN cassette contained a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene (tACE-Cre). The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into FVB/N blastocysts and chimeric males were bred to C57BL/6J females. The donating investigator reported that the resulting Foxp3GFP mice were maintained on a C57BL/6J background (see SNP note below). Upon arrival mice at The Jackson Laboratory these mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	Foxp3, forkhead box P3, mouse, laboratory
Site of Expression	EGFP expression is observed in FOXP3+ regulatory T cells.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wang Y; Kissenpfennig A; Mingueneau M; Richelme S; Perrin P; Chevrier S; Genton C; Lucas B; DiSanto JP; Acha-Orbea H; Malissen B; Malissen M. 2008. Th2 lymphoproliferative disorder of LatY136F mutant mice unfolds independently of TCR-MHC engagement and is insensitive to the action of Foxp3+ regulatory T cells. J Immunol 180(3):1565-75PubMed: 18209052 MGI: J:131351

View All References

Genetics

Foxp3^tm1Mal

Allele Symbol: Foxp3^tm1Mal

Allele Name	targeted mutation 1, Bernard Malissen
Allele Type	Targeted (Reporter)
Allele Synonym(s)	Foxp3.LuciDTR-4; FOXP3EGFP; Foxp3-GDL
Gene Symbol and Name	Foxp3, forkhead box P3
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	Foxp3, forkhead box P3, mouse, laboratory
Site of Expression	EGFP expression is observed in FOXP3+ regulatory T cells.
Strain of Origin	B6.Cg-Thy1^a
Chromosome	X
Molecular Note	An IRES-EGFP cassette and a floxed cre-neo autodeleter casstte were inserted into exon 11, which lies in the downstream untranslated region. Passage through the male germ line-derived ES cells resulted in deletion of the cre-neo autodeleter cassette. The presence of the EGFP cassette does not interfer with expression of the gene locus. EGFP expression occurs in Foxp3⁺ regulatory T cells.
Mutations Made By	Bernard Malissen, Centre d'immunologie de Marseille Luminy

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker

Mammalian Phenotype Terms by Genotype

References

Wang Y; Kissenpfennig A; Mingueneau M; Richelme S; Perrin P; Chevrier S; Genton C; Lucas B; DiSanto JP; Acha-Orbea H; Malissen B; Malissen M. 2008. Th2 lymphoproliferative disorder of LatY136F mutant mice unfolds independently of TCR-MHC engagement and is insensitive to the action of Foxp3+ regulatory T cells. J Immunol 180(3):1565-75PubMed: 18209052 MGI: J:131351

Additional - Foxp3^tm1Mal related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Foxp3
- Genotyping resources and troubleshooting
Breeding Considerations

This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females may be bred with hemizygous males.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Hemizygote
Citation
When using the Foxp3EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #018628 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Foxp3<tm1Mal>

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Foxp3<tm1Mal>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Foxp3<tm1Mal>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Foxp3<tm1Mal>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.Cg-Foxp3<tm1Mal>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Foxp3EGFP

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Foxp3tm1Mal

Allele Symbol: Foxp3tm1Mal

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Foxp3tm1Mal related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Foxp3^tm1Mal

Allele Symbol: Foxp3^tm1Mal

Additional - Foxp3^tm1Mal related