The Tgfb3 gene is replaced with a Tgfb1 cDNA in these mice, abolishing Tgfb3 expression. TGFB1 expression partially rescues the clefting phenotype associated with Tgfb3 disruption. These mice may be suitable for use in studies examining the isoform-specific roles of transforming growth factors.
Dr. Vesa Kaartinen, University of Michigan
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout, Inserted expressed sequence) | Tgfb3 | transforming growth factor, beta 3 |
In this knock in/out mutant, the transforming growth factor, beta 3 (Tgfb3) coding region was replaced with a transforming growth factor, beta 1 (Tgfb1) cDNA, abolishing Tgfb3 expression. TGFB isoforms are important regulators of cell growth, cell differentiation, apoptosis, and cellular homeostasis. TGFB3 is expressed in the prefusion palatal epithelium and KO models display a cleft of the secondary palate, lung hypoplasia, respiratory distress, hemothorax, and neonatal lethality. When TGFB1 is knocked into the TGFB3 coding region, the clefting phenotype is partially rescued. These mutant mice show partial fusion of the secondary palate. They exhibit a reduction in apoptosis in the midline epithelium during fusion of the palatal shelves, causing anterior and posterior regions of the palate to not fuse. Homozygous mice are viable but do not survive past 2 weeks of age due to failure to feed.
A targeting vector was designed to insert transforming growth factor, beta 1 (Tgfb1) cDNA, followed by a polyadenylation sequence and a loxP-flanked neomycin resistance cassette, into the transforming growth factor, beta 3 (Tgfb3) coding region. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to B6xCBA F1 females to generate a colony of Tgfb1-KI mice. These mice were maintained on a mixed genetic background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | Tgfb1, transforming growth factor, beta 1, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 2, Vesa Kaartinen |
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Allele Type | Targeted (Null/Knockout, Inserted expressed sequence) |
Allele Synonym(s) | Tgfb1-KI |
Gene Symbol and Name | Tgfb3, transforming growth factor, beta 3 |
Gene Synonym(s) | |
Expressed Gene | Tgfb1, transforming growth factor, beta 1, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 12 |
Molecular Note | A vector containing Tgfb1 cDNA followed by a pgk polyA sequence and a loxP flanked neomycin resistance cassette was inserted into the ATG start site of the targeted gene. Quantitative RT-PCR of RNA isolated from the tips of the palatal shelves demonstrates high Tgfb1 expression but no Tgfb3 expression in homozygotes. |
Mutations Made By | Dr. Vesa Kaartinen, University of Michigan |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. The donating investigator reports that homozygous mice are viable but do not survive past 2 weeks of age due to failure to feed.
When using the STOCK Tgfb3tm2(Tgfb1)Vk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018624 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Tgfb3<tm2(Tgfb1)Vk> |
Frozen Mouse Embryo | STOCK Tgfb3<tm2(Tgfb1)Vk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tgfb3<tm2(Tgfb1)Vk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tgfb3<tm2(Tgfb1)Vk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tgfb3<tm2(Tgfb1)Vk>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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