This GFAP-DNSynCAM1 transgenic line expresses a dominant-negative form of the CADM1 protein that lacks the intracellular domain, exhibits hyperactivity, increased aggression, and has applications in studies of attention-deficit hyperactive disorder (ADHD).
Sergio R. Ojeda, Oregon National Primate Research Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Dominant negative, Inserted expressed sequence) |
GFAP-DNSynCAM1 transgenic mice express a dominant-negative form of the CADM1 protein that lacks the intracellular domain in an astrocyte-specific fashion, under the control of the human GFAP, glial fibrillary acidic protein, promoter. The transgene is expressed in the brain, and the expression pattern of the transgene mimics the endogenous gene expression pattern, as determined by immunohistochemical and Western blot analysis of EGFP. Transgenic mice on the FVB background display hyperactivity increased spontaneous locomoter activity in the open field, reduced anxiety in the zero maze, altered response to d,l-amphetamine and increased aggression. Behavioral tests reveal that transgenic mice, on the mixed B6;FVB background, have a disrupted diurnal pattern with increased total activity and reduced resting times during the light phase. Mice hemizygous for the transgenic insert are viable and fertile, although female transgenic mice have delayed puberty onset (by approximately 6 days), disrupted estrous cycles, and reduced fecundity (transgenic litter size approximately 65% of wildtype control litter size). The Donating Investigator reports that the strain cannot be maintained as homozygotes. There is no difference between the phenotype exhibited by homozygotes compared to hemizygotes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing a mutant mouse Cadm1 (cell adhesion molecule 1) sequence encoding a dominant-negative form of the protein that lacks the intracellular domain (EGFP replaced the sequence encoding the intracellular domain), under the control of the human GFAP, glial fibrillary acidic protein, promoter, was injected into fertilized FVB/NJ mouse eggs. Founder line 42 was subsequently established. The mice were maintained the FVB/N background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Cadm1, cell adhesion molecule 1, mouse, laboratory |
Site of Expression | A dominant-negative form of the CADM1 and GFP are expressed in the brain. |
Allele Name | Transgene insertion 42, Sergio R Ojeda |
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Allele Type | Transgenic (Reporter, Dominant negative, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(GFAP-Cadm1/EGFP)42Oje, Transgene insertion 42, Sergio R Ojeda |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Cadm1, cell adhesion molecule 1, mouse, laboratory |
Site of Expression | A dominant-negative form of the CADM1 and GFP are expressed in the brain. |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | Mouse Cadm1 sequence encoding a dominant-negative form of the protein lacking the intracellular domain (EGFP replaced the sequence encoding the intracellular domain) was placed under the control of the human GFAP promoter. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator reports that the strain cannot be maintained as homozygous. Transgenic female mice have delayed puberty onset, disrupted estrous cycles, and reduced fecundity.
When using the FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018548 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(GFAP-Cadm1/EGFP)42Oje |
Frozen Mouse Embryo | FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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