| Allele Name | transgene insertion E9, Dawn S Chandler |
| Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | Tg hSMN2 E9 |
| Gene Symbol and Name | Tg(CAG-Bgeo,-SMN2)E9Dscd, transgene insertion E9, Dawn S Chandler |
| Gene Synonym(s) | |
| Promoter | CMV, cytomegalovirus, human |
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| Expressed Gene | SMN2, survival of motor neuron 2, centromeric, human |
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| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
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| Site of Expression | lacZ expression is seen in all cells prior to Cre recombination. After cre excision of the floxed- lacZ-neo cassette, human SMN2 is expressed in cre-expressing tissues. |
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| Strain of Origin | 129S6/SvEvTac |
| Chromosome | UN |
| Molecular Note | The pCCALL2/hSMN2 transgene was designed with, from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (originally from the pCAGG vector), a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA signal), the 1.37 kbp full-length human SMN2 cDNA sequence including all exons (1-8) from the start codon to the stop codon, and a rabbit polyA signal. The 1.37 kbp human SMN2 cDNA sequence (encoding exons 1-8) was originally derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Lines A9 and E9 was generated. |
| Mutations Made By | Dawn Chandler, The Ohio State University School of Med. |
