Overview

This Pax2^Egfp knock-in/knockout strain has its Pax2 gene disrupted by an inserted EGFP sequence. These mice may be useful in applications related to the study of the developing intermediate mesoderm (kidney) and male/female urogenital epithelial structures, renal coloboma syndrome, and renal-specific stem cells.

Donating Investigator

Gregory R Dressler, University of Michigan

Genetic overview

Genetic Background	Generation

Pax2^tm1.1Gdr

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Pax2	paired box 2

View Genetics

Research Applications

Neurobiology Research
Sensorineural Research
Developmental Biology Research
Internal/Organ Research
Research Tools
Reproductive Biology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

The Pax2^Egfp knock-in/knockout allele has an EGFP with polyA signal upstream of the endogenous paired box gene 2 (Pax2) translational start site; both abolishing endogenous gene function and placing EGFP expression under direction of the Pax2 promoter/enhancer regions. All mice homozygous for the Pax2^Egfp allele exhibit exencephaly, complete renal agenesis, and die at birth. The donating investigator did not assess if any eye/ear abnormalities were present. Mice heterozygous for the Pax2^Egfp allele are viable, fertile, and phenotypically indistinguishable from wildtype littermate controls (do not exhibit kidney structural defects). In both heterozygous and homozygous mice, EGFP expression (direct fluorescence) is observed in a spatial and temporal pattern consistent with the expected expression of endogenous Pax2. The EGFP expression of Pax2^Egfp heterozygous mice is described in detail below.
At embryonic day (E)9.5, EGFP expression is observed in optic cup, otic vesicle, the midbrain-hindbrain junction, and developing nephric duct. By E10.5, EGFP positive cells of the intermediate mesoderm are segregated into epithelial duct cells and adjacent mesenchyme. By E11.5, EGFP is seen in the ureteric bud and condensing mesenchyme of the metanephric kidney, as well as Pax2-positive neurons that developed at this time along the entire neural tube. At later stages of development, kidney epithelia, ureters, and all the male and female specific epithelia derived from the intermediate mesoderm are EGFP positive. In newborn mice, EGFP expression is strong in the entire nephrogenic zone (nephron differentiation is still ongoing). In adult kidneys, EGFP expression is observed in the renal papilla. When comparing EGFP expression of mice heterozygous for the Pax2^Egfp knockout allele (Stock No. 018438) to mice heterozygous for the Pax2^Eneo hypomorphic allele (Stock No. 018437), there is no qualitative difference in the temporal and spatial pattern of EGFP, although the intensity of EGFP is higher in Pax2^Egfp heterozygotes compared to Pax2^Eneo heterozygotes.

Development

The Pax2-Eneo targeting vector was designed to insert an enhanced green fluorescent protein sequence (EGFP; with polyA signal) and frt-flanked PGK-neo cassette upstream of the translational start site in exon 1 of the paired box gene 2 gene (Pax2). The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females to establish the Pax2^Eneo colony. The donating investigator reports that to remove the frt-flanked PGK-neo cassette, some Pax2^Eneo mice were bred to C57BL/6 animals expressing FLP recombinase. The resulting mice with EGFP and single frt site remaining upstream of the Pax2 coding region were identified. These Pax2^Egfp mice were subsequently bred to wildtype mice for several generations (and the FLP gene was removed). Pax2^Egfp mice on a mixed C57BL/6;129 genetic background were then sent to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish The Jackson Laboratory Repository colony.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	At embryonic day (E)9.5, EGFP expression is observed in optic cup, otic vesicle, the midbrain-hindbrain junction, and developing nephric duct. By E10.5, EGFP positive cells of the intermediate mesoderm are segregated into epithelial duct cells and adjacent mesenchyme. By E11.5, EGFP is seen in the ureteric bud and condensing mesenchyme of the metanephric kidney, as well as Pax2-positive neurons that developed at this time along the entire neural tube. At later stages of development, kidney epithelia, ureters, and all the male and female specific epithelia derived from the intermediate mesoderm are EGFP positive. In newborn mice, EGFP expression is strong in the entire nephrogenic zone (nephron differentiation is still ongoing). In adult kidneys, EGFP expression is observed in the renal papilla.

Expressed Gene

GFP, Green Fluorescent Protein,

Site of Expression

At embryonic day (E)9.5, EGFP expression is observed in optic cup, otic vesicle, the midbrain-hindbrain junction, and developing nephric duct. By E10.5, EGFP positive cells of the intermediate mesoderm are segregated into epithelial duct cells and adjacent mesenchyme. By E11.5, EGFP is seen in the ureteric bud and condensing mesenchyme of the metanephric kidney, as well as Pax2-positive neurons that developed at this time along the entire neural tube. At later stages of development, kidney epithelia, ureters, and all the male and female specific epithelia derived from the intermediate mesoderm are EGFP positive. In newborn mice, EGFP expression is strong in the entire nephrogenic zone (nephron differentiation is still ongoing). In adult kidneys, EGFP expression is observed in the renal papilla.

Control Suggestions

Wild-type from the colony

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Soofi A; Levitan I; Dressler GR. 2012. Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneys. Dev Biol 365(1):241-50PubMed: 22410172 MGI: J:184878

View All References

Genetics

Pax2^tm1.1Gdr

Allele Symbol: Pax2^tm1.1Gdr

Allele Name	targeted mutation 1.1, Gregory R Dressler
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Pax2^Egfp
Gene Symbol and Name	Pax2, paired box 2
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	At embryonic day (E)9.5, EGFP expression is observed in optic cup, otic vesicle, the midbrain-hindbrain junction, and developing nephric duct. By E10.5, EGFP positive cells of the intermediate mesoderm are segregated into epithelial duct cells and adjacent mesenchyme. By E11.5, EGFP is seen in the ureteric bud and condensing mesenchyme of the metanephric kidney, as well as Pax2-positive neurons that developed at this time along the entire neural tube. At later stages of development, kidney epithelia, ureters, and all the male and female specific epithelia derived from the intermediate mesoderm are EGFP positive. In newborn mice, EGFP expression is strong in the entire nephrogenic zone (nephron differentiation is still ongoing). In adult kidneys, EGFP expression is observed in the renal papilla.
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	19
Molecular Note	An EGFP and FRT-flanked neo cassette were inserted upstream of the coding sequence in exon 1. Flp-mediated recombination removed the neo cassette.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Hearing Defects
- Fluorescent protein expression in neural tissue
- Neurodevelopmental Defects
Sensorineural Research
- Hearing Defects
- Eye Defects
  - Optic Nerve Coloboma
Developmental Biology Research
- Perinatal Lethality
  - Homozygous
- Eye Defects
- Mesodermal Defects
- Internal/Organ Defects
  - kidney
  - urogenital
Internal/Organ Research
- Kidney Defects
  - nephronophthisis/medullary cystic disease model
Research Tools
- Fluorescent Proteins
- Developmental Biology Research
- Genetics Research
  - Tissue/Cell Markers: kidney specific marker
- Reproductive Biology Research
Reproductive Biology Research
- Developmental Defects Affecting Gonads

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Pax2^tm1.1Gdr/Pax2^tm1.1Gdr
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

mortality/aging

neonatal lethality, complete penetrance

nervous system phenotype

exencephaly

renal/urinary system phenotype

abnormal kidney development
- E10.5 kidney epithelial cells exhibit loss of polarity compared with wild-type cells

absent kidney

References

Soofi A; Levitan I; Dressler GR. 2012. Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneys. Dev Biol 365(1):241-50PubMed: 22410172 MGI: J:184878

Additional - Pax2^tm1.1Gdr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Pax2alternate1
- Separated PCR:Pax2alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

All mice homozygous for the Pax2^Egfp allele exhibit exencephaly, complete renal agenesis, and die at birth. When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129-Pax2^tm1.1Gdr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018438 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wildtype for Pax2<tm1.1Gdr>

Related Products and Services

Frozen Mouse Embryo	B6;129-Pax2<tm1.1Gdr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129-Pax2<tm1.1Gdr>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129-Pax2<tm1.1Gdr>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129-Pax2<tm1.1Gdr>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Pax2tm1.1Gdr

Allele Symbol: Pax2tm1.1Gdr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Pax2tm1.1Gdr/Pax2tm1.1Gdrinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

mortality/aging

nervous system phenotype

renal/urinary system phenotype

References

Additional - Pax2tm1.1Gdr related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Pax2^tm1.1Gdr

Allele Symbol: Pax2^tm1.1Gdr

Genotype: Pax2^tm1.1Gdr/Pax2^tm1.1Gdr
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - Pax2^tm1.1Gdr related