These autophagy-related beclin 1 (Becn1) knockout mice exhibit late onset lung carcinomas, hepatocellular carcinomas and lymphomas and may be useful for studying autophagy, negative cell growth and tumor suppression.
Beth Levine, University of Texas Southwestern Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Becn1 | beclin 1, autophagy related |
Monoallelic deletion of the autophagy related beclin 1 (Becn1) gene is associated with 40-75% of human breast, ovarian and prostate cancers. 30% of mice heterozygous for the Becn1 allele exhibit tumors by 13-18 months of age. Tumor types include: lung carcinomas, hepatocellular carcinomas and lymphomas. In addition, mice develop hepatitis B virus-induced neoplasia, increased cellular proliferation and reduced autophagy. Homozygous deletion of the targeted allele results in embryonic lethality. Of note, mice on a C57BL/6 background have a brownish (agouti) coat color which is believed to be a result of the effect of the Becn1 mutation on melanogenesis. These mice may be useful for studies involving autophagy and negative cell growth and tumor suppression.
A targeting vector was designed to replace exons 1 and 2 with a neomycin resistance (neo) cassette. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6. Offspring were backcrossed to C57BL/6 for 50 generations, however, mice retain a brownish (agouti) coat color, which is believed to be a result of the role of Becn1 in melanogenesis. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Beth Levine |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Atg6-; beclin 1- |
Gene Symbol and Name | Becn1, beclin 1, autophagy related |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 11 |
Molecular Note | Exons 1 and 2 were replace with a neomycin selection cassette inserted by homologous recombination. |
Mutations Made By | Beth Levine, University of Texas Southwestern Medical Center |
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable. Of note, mice on a C57BL/6 background have a brown coat color which is believed to be a result of the Becn1 mutation on melanogenesis.
When using the Atg6- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018429 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Becn1<tm1Blev> |
Frozen Mouse Embryo | B6.129X1-Becn1<tm1Blev>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Becn1<tm1Blev>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Becn1<tm1Blev>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Becn1<tm1Blev>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.