In this strain a NEO cassette replaces the coding region of the Bc1 (brain cytoplasmic RNA 1) gene. These mice exhibit neuronal hyperexcitability and have applications in studies related to synaptic dysregulation.
Jun Zhong, State University of New York
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Bc1 | brain cytoplasmic RNA 1 |
Brain cytoplasmic RNAs regulate translation initiation. The neuronal brain cytoplasmic RNA 1, gene (Bc1), is involved in synaptic plasticity. These mice carry a knockout allele for the Bc1 gene, in which the entire coding region has been replaced by a NEO cassette. On the mixed B6;129 background, homozygotes are more susceptible to audiogenic seizures due to neuronal hyperexcitability. Most homozygotes (84% on the B6;129 background) exposed to auditory stimulation (120dB alarm) exhibit generalized clonic-tonic convulsive seizures after 2 minutes of exposure, but recover after approximately 1 minute. Approximately a quarter of the mice die during the seizures. Homozygotes display reduced exploration behavior and increased anxiety. The Donating Investigator reports that on the C57BL/6 congenic background, homozygotes aged 18 - 21 days, exhibit excessive grooming behavior, and are not susceptible to audiogenic seizures.
Mice that are homozygous for the targeted mutation are viable and fertile. No transcript is detected by Northern blot analysis of brain, testes, and liver. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
When crossed to mice homozygous for the Fmr1tm1Cgr targeted mutation
(Stock No. 003024), the double mutant mice exhibit CA3 pyramidal
cell hyperexcitability that is more severe than observed in the single mutant lines.
A targeting vector containing a PGK-NEO cassette was used to disrupt the entire coding region of the gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. Heterozygotes were intercrossed to generate homozygotes. The mice were then backcrossed to C57BL/6 for 15 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Jurgen Brosius |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Bc1, brain cytoplasmic RNA 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | The endogenous gene was replaced with a neomycin selection cassette inserted by homologous recombination. Northern blot analysis confirmed the absence of transcript in homozygous mutant mice. |
Mutations Made By | Jun Zhong, State University of New York |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Bc1tm1Jbro/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018417 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bc1<tm1Jbro> |
Frozen Mouse Embryo | B6.129-Bc1<tm1Jbro>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Bc1<tm1Jbro>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Bc1<tm1Jbro>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Bc1<tm1Jbro>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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