Overview

Also Known As:Irf8^-

Mice homozygous for the Irf8 knockout allele (Irf8^-/-) may be useful in studying immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.

Donating Investigator

IMR Colony, The Jackson Laboratory

Herbert C. Morse III, Laboratory of Immunopathology, NIAID, NI

Andrew P McMahon, University of Southern California

Genetic overview

Genetic Background	Generation

Irf8^tm1.2Hm

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Irf8	interferon regulatory factor 8

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Hematological Research
Research Tools
Virology Research
Developmental Biology Research
Cell Biology Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this Irf8 knockout allele (Irf8^-/-) may be expected to to exhibit some or all of the phenotype published for other Irf8 null mutations on both a C57BL/6J-congenic and mixed genetic background. For example, other Ifr8 null mice are viable and fertile as young animals. Homozygotes are immunodeficient (abnormal populations of B cells, T cells, granulocytes and macrophages) and develop a syndrome similar to human chronic myeloid leukemia. Acute onset begins by 10 weeks of age, progresses through chronic stage (~20 weeks of age), and significant mortality by ~50 weeks of age.

In an attempt to offer knockout versions of conditional alleles, The Jackson Laboratory Repository bred floxed mice (Stock No. 014175) with germline-expressing Cre recombinase mice (Stock No. 008454). The resulting animals with pan deletion of the floxed exons (Irf8^-/-) are available as Stock No. 018298. It should be noted that the phenotype of these homozygous knockout mice could vary from that published/described for other knockout mice established in different laboratories or on different genetic backgrounds. We may modify our strain description if necessary as results become available.

Development

The Irf8^f targeted mutation was designed by Dr. Herbert C. Morse III (National Institute of Allergy and Infectious Diseases) to have loxP sites on either side of exon 2 of the interferon regulatory factor 8 gene. In 2011, Irf8^f mice on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository as Stock No. 014175. Upon arrival, some Irf8^f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon 2 were identified and then selectively bred to C57BL/6J inbred mice (Stock No. 000664) to remove the Cre transgene. The resulting mice, heterozygous for the Irf8-deficient allele (Irf8^-), were subsequently bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice to establish Stock No. 018298.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME UnpublishedMGI: J:101977

View All References

Genetics

Irf8^tm1.2Hm

Allele Symbol: Irf8^tm1.2Hm

Allele Name	targeted mutation 1.2, Herbert Morse III
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Irf8, interferon regulatory factor 8
Gene Synonym(s)
Strain of Origin	B6.Cg-Thy1^a
Chromosome	8
Molecular Note	Exon 2 was floxed. Cre-mediated recombination removed exon 2.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - multiple immune defects
  - B cell defects
  - B cell deficiency
  - Macrophage defects
  - defects in humoral immune responses
  - B and T cell deficiency
- Immunodeficiency Associated with Other Defects
- Intracellular Signaling Molecules
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - hematopoietic development
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - T cell deficiency
- Thymus Defects
  - B and T cell deficient
Hematological Research
- Hematopoietic Defects
- Immunological Defects
  - B and T cell deficiency
Research Tools
- Cancer Research
  - B cell deficiency
  - T cell deficiency
  - Leukemia
- Immunology, Inflammation and Autoimmunity Research
  - B and T cell deficiency
  - T cell deficiency
  - B cell deficiency
  - Macrophage Deficiency
Virology Research
- B and T Cell Deficiency
Developmental Biology Research
- Internal/Organ Defects
  - hematopoietic defects
- Lymphoid Tissue Defects
  - hematopoietic defects
Cell Biology Research
- Signal Transduction
Cancer Research
- Chronic Myelogenous Leukemia
- Increased Tumor Incidence
  - Leukemia: lymphocytic
  - Leukemia

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Irf8^tm1.2Hm/Irf8⁺
involves: C57BL/6

mammalian phenotype

no phenotypic analysis

References

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME UnpublishedMGI: J:101977

Additional - Irf8^tm1.2Hm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Irf8
- Standard PCR:Irf8
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664).
- Additional Breeding and Husbandry Support
Citation
When using the Irf8^- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018298 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wildtype for Irf8<tm1.2Hm>

Related Products and Services

Frozen Mouse Embryo	B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Irf8-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Irf8tm1.2Hm

Allele Symbol: Irf8tm1.2Hm

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Irf8tm1.2Hm/Irf8+involves: C57BL/6

mammalian phenotype

References

Additional - Irf8tm1.2Hm related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:Irf8^-

Irf8^tm1.2Hm

Allele Symbol: Irf8^tm1.2Hm

Genotype: Irf8^tm1.2Hm/Irf8⁺
involves: C57BL/6

Additional - Irf8^tm1.2Hm related