B6(Cg)-Irf8^tm1.2Hm/J

Stock number: 018298
Product name: Irf8^-
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

Mice homozygous for the Irf8 knockout allele (Irf8^-/-) may be useful in studying immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.

Detailed description

Mice homozygous for this Irf8 knockout allele (Irf8^-/-) may be expected to to exhibit some or all of the phenotype published for other Irf8 null mutations on both a C57BL/6J-congenic and mixed genetic background. For example, other Ifr8 null mice are viable and fertile as young animals. Homozygotes are immunodeficient (abnormal populations of B cells, T cells, granulocytes and macrophages) and develop a syndrome similar to human chronic myeloid leukemia. Acute onset begins by 10 weeks of age, progresses through chronic stage (~20 weeks of age), and significant mortality by ~50 weeks of age.

In an attempt to offer knockout versions of conditional alleles, The Jackson Laboratory Repository bred floxed mice (Stock No. 014175) with germline-expressing Cre recombinase mice (Stock No. 008454). The resulting animals with pan deletion of the floxed exons (Irf8^-/-) are available as Stock No. 018298. It should be noted that the phenotype of these homozygous knockout mice could vary from that published/described for other knockout mice established in different laboratories or on different genetic backgrounds. We may modify our strain description if necessary as results become available.

Development

The Irf8^f targeted mutation was designed by Dr. Herbert C. Morse III (National Institute of Allergy and Infectious Diseases) to have loxP sites on either side of exon 2 of the interferon regulatory factor 8 gene. In 2011, Irf8^f mice on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository as Stock No. 014175. Upon arrival, some Irf8^f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon 2 were identified and then selectively bred to C57BL/6J inbred mice (Stock No. 000664) to remove the Cre transgene. The resulting mice, heterozygous for the Irf8-deficient allele (Irf8^-), were subsequently bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice to establish Stock No. 018298.

Allele

Symbol: Irf8^tm1.2Hm
Name: targeted mutation 1.2, Herbert Morse III
MGI accession ID: MGI:5491303
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Irf8
Marker name: interferon regulatory factor 8
Marker synonyms: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; Myls
Chromosome: 8
Strain of origin: B6.Cg-Thy1^a
Molecular note: Exon 2 was floxed. Cre-mediated recombination removed exon 2.