Mice homozygous for the Irf8 knockout allele (Irf8-/-) may be useful in studying immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.
IMR Colony, The Jackson Laboratory
Herbert C. Morse III, Laboratory of Immunopathology, NIAID, NI
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Irf8 | interferon regulatory factor 8 |
Mice homozygous for this Irf8 knockout allele (Irf8-/-) may be expected to to exhibit some or all of the phenotype published for other Irf8 null mutations on both a C57BL/6J-congenic and mixed genetic background. For example, other Ifr8 null mice are viable and fertile as young animals. Homozygotes are immunodeficient (abnormal populations of B cells, T cells, granulocytes and macrophages) and develop a syndrome similar to human chronic myeloid leukemia. Acute onset begins by 10 weeks of age, progresses through chronic stage (~20 weeks of age), and significant mortality by ~50 weeks of age.
In an attempt to offer knockout versions of conditional alleles, The Jackson Laboratory Repository bred floxed mice (Stock No. 014175) with germline-expressing Cre recombinase mice (Stock No. 008454). The resulting animals with pan deletion of the floxed exons (Irf8-/-) are available as Stock No. 018298. It should be noted that the phenotype of these homozygous knockout mice could vary from that published/described for other knockout mice established in different laboratories or on different genetic backgrounds. We may modify our strain description if necessary as results become available.
The Irf8f targeted mutation was designed by Dr. Herbert C. Morse III (National Institute of Allergy and Infectious Diseases) to have loxP sites on either side of exon 2 of the interferon regulatory factor 8 gene. In 2011, Irf8f mice on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository as Stock No. 014175. Upon arrival, some Irf8f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon 2 were identified and then selectively bred to C57BL/6J inbred mice (Stock No. 000664) to remove the Cre transgene. The resulting mice, heterozygous for the Irf8-deficient allele (Irf8-), were subsequently bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice to establish Stock No. 018298.
Allele Name | targeted mutation 1.2, Herbert Morse III |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Irf8, interferon regulatory factor 8 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 8 |
Molecular Note | Exon 2 was floxed. Cre-mediated recombination removed exon 2. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664).
When using the Irf8- mouse strain in a publication, please cite the originating article(s) and include JAX stock #018298 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Irf8<tm1.2Hm> |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.2Hm>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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