These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the mouse Drd1a promoter. These Drd1BAC-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in D1A dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.
Robert G. MacKenzie, Wayne State University Sch. of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the mouse Drd1a, dopamine receptor D1A, promoter. Hemizygous mice are viable and fertile. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in D1A dopamine receptor subtype-expressing neurons is induced with administration of the tetracycline analog, doxycycline (dox). These Drd1BAC-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in D1A dopamine receptor subtype-expressing neurons.
When bred to a beta-galactosidase reporter strain (tetO-lacZ), the resulting double mutant mice exhibit a beta-galactosidase activity pattern that mimics the endogenous Drd1a expression pattern.
A mouse bacterial artificial chromosome (BAC), RP23-47M2, containing the Drd1a gene, was modified by insertion of reverse tetracycline transactivator gene (rtTA) sequence, such that the Drd1a promoter drives expression of the rtTA. The BAC was linearized and microinjected into the pronucleus of (C57BL/6 X SJL)F2 fertilized eggs. Founder line A was subsequently established. The mice were then bred to mice on a mixed C57BL/6, SJL and FVB background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | When mated to a transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in D1A dopamine receptor subtype-expressing neurons is induced with administration of the tetracycline analog, doxycycline (dox). |
Allele Name | transgene insertion A, Robert MacKenzie |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Drd1a-rtTA)ARgmk, transgene insertion A, Robert MacKenzie |
Gene Synonym(s) | |
Promoter | Drd1, dopamine receptor D1, mouse, laboratory |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | When mated to a transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in D1A dopamine receptor subtype-expressing neurons is induced with administration of the tetracycline analog, doxycycline (dox). |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | A mouse bacterial artificial chromosome (BAC), RP23-47M2, containing the Drd1a gene, was modified by insertion of reverse tetracycline transactivator gene (rtTA) sequence, such that the Drd1a promoter drives expression of the rtTA. Founder line A was subsequently established. |
Mutations Made By | Robert MacKenzie, Wayne State University Sch. of Medicine |
When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make this strain homozygous.
When using the STOCK Tg(Drd1a-rtTA)ARgmk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018156 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Drd1a-rtTA)ARgmk |
Frozen Mouse Embryo | STOCK Tg(Drd1a-rtTA)ARgmk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Drd1a-rtTA)ARgmk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Drd1a-rtTA)ARgmk/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Drd1a-rtTA)ARgmk/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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