STOCK Tg(Drd1a-rtTA)ARgmk/J

Stock number: 018156
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the mouse Drd1a promoter. These Drd1BAC-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in D1A dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.

Detailed description

These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the mouse Drd1a, dopamine receptor D1A, promoter. Hemizygous mice are viable and fertile. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in D1A dopamine receptor subtype-expressing neurons is induced with administration of the tetracycline analog, doxycycline (dox). These Drd1BAC-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in D1A dopamine receptor subtype-expressing neurons.

When bred to a beta-galactosidase reporter strain (tetO-lacZ), the resulting double mutant mice exhibit a beta-galactosidase activity pattern that mimics the endogenous Drd1a expression pattern.

Development

A mouse bacterial artificial chromosome (BAC), RP23-47M2, containing the Drd1a gene, was modified by insertion of reverse tetracycline transactivator gene (rtTA) sequence, such that the Drd1a promoter drives expression of the rtTA. The BAC was linearized and microinjected into the pronucleus of (C57BL/6 X SJL)F2 fertilized eggs. Founder line A was subsequently established. The mice were then bred to mice on a mixed C57BL/6, SJL and FVB background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Drd1a-rtTA)ARgmk
Name: transgene insertion A, Robert MacKenzie
MGI accession ID: MGI:5317667
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Drd1a-rtTA)ARgmk
Marker name: transgene insertion A, Robert MacKenzie
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: When mated to a transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in D1A dopamine receptor subtype-expressing neurons is induced with administration of the tetracycline analog, doxycycline (dox).
Molecular note: A mouse bacterial artificial chromosome (BAC), RP23-47M2, containing the Drd1a gene, was modified by insertion of reverse tetracycline transactivator gene (rtTA) sequence, such that the Drd1a promoter drives expression of the rtTA. Founder line A was subsequently established.