B6;129-Igs1^{tm4(CAG-Bgeo,-Vangl2)Nat}/J

Stock number: 018139
Research areas: Research Tools

Description

This Ubb (ubiquitin B) knock-in strain gives consitutive expression of nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, Vangl2 (vang-like 2 (van gogh, Drosophila)) protein with 3xHA (hemagglutinin) tag is expressed.

Detailed description

This Ubb (ubiquitin B) knock-in strain gives consitutive expression of nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, the NLS-beta-geo coding region is excised and the downstream Vangl2 (vang-like 2 (van gogh, Drosophila)) protein with 3xHA (hemagglutinin) tag at the N-terminus is expressed. This reporter can be activated by cre recombinase in any cell in the body at any time in development. The result is target gene recombination.

Development

The following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter, loxP site, beta-geo, 3 transcription sites, loxP site, 3HA-Vangl2, rabbit beta-globin 3' UTR. This strain was backcrossed to C57BL/6J three times by the donating laboratory.

Allele

Symbol: Igs1^{tm4(CAG-Bgeo,-Vangl2)Nat}
Name: targeted mutation 4, Jeremy Nathans
MGI accession ID: MGI:5314813
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; No functional change
Marker symbol: Igs1
Marker name: intergenic site 1
Chromosome: 11
Strain of origin: 129
Expressed genes: Bgeo,fusion of beta-galactosidase and neomycin phosphotransferase genes,E. coli
Site of expression: Consitutive expression of the nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo).
Molecular note: The following elements were inserted, in order, 2 kb 5' of the gene by targeted recombination: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo cassette, 3 transcription stop sites, a loxP site, Vangl2 with 3 HA-tags, and rabbit beta-globin 3' UTR.