This Ubb (ubiquitin B) knock-in strain gives consitutive expression of nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, Vangl2 (vang-like 2 (van gogh, Drosophila)) protein with 3xHA (hemagglutinin) tag is expressed.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence, No functional change) | Igs1 | intergenic site 1 |
This Ubb (ubiquitin B) knock-in strain gives consitutive expression of nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, the NLS-beta-geo coding region is excised and the downstream Vangl2 (vang-like 2 (van gogh, Drosophila)) protein with 3xHA (hemagglutinin) tag at the N-terminus is expressed. This reporter can be activated by cre recombinase in any cell in the body at any time in development. The result is target gene recombination.
The following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter, loxP site, beta-geo, 3 transcription sites, loxP site, 3HA-Vangl2, rabbit beta-globin 3' UTR. This strain was backcrossed to C57BL/6J three times by the donating laboratory.
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
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Site of Expression | Consitutive expression of the nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo). |
Allele Name | targeted mutation 4, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence, No functional change) |
Allele Synonym(s) | Iis1tm4(CAG-Bgeo,-Vangl2)Nat; Ubbtm1(3HA-Vangl2)Nat; Ubbtm4Nat |
Gene Symbol and Name | Igs1, intergenic site 1 |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Site of Expression | Consitutive expression of the nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo). |
Strain of Origin | 129 |
Chromosome | 11 |
Molecular Note | The following elements were inserted, in order, 2 kb 5' of the gene by targetedrecombination: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo cassette, 3 transcription stop sites, a loxP site, Vangl2 with 3 HA-tags, and rabbit beta-globin 3' UTR. |
Heterozygotes are viable and fertile, but homozygotes are unhealthy.
When using the B6;129-Igs1tm4(CAG-Bgeo,-Vangl2)Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018139 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Iis1<tm4(CAG-Bgeo,-Vangl2)Nat> |
Frozen Mouse Embryo | B6;129-Igs1<tm4(CAG-Bgeo -Vangl2)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Igs1<tm4(CAG-Bgeo -Vangl2)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Igs1<tm4(CAG-Bgeo -Vangl2)Nat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Igs1<tm4(CAG-Bgeo -Vangl2)Nat>/J Frozen Embryo | $3373.50 |
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