This strain may be useful for generating spatial and temporal conditional mutants for applications related to genetically complex seizure disorders. Inserted loxP sites flank exon 1 of the Clef4 gene, a brain specific RNA binding protein.
Wayne Frankel, Columbia University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Celf4 | CUGBP, Elav-like family member 4 |
CELF4 (CUGBP, Elav-like family member 4) is a brain specific RNA binding protein. CELF4 has widespread expression in early development, but is limited to the central nervous system in adults. These mutant mice possess loxP sites flanking exon 1 of the (Clef4) gene. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in the cre-expressing tissues.
For example, when bred to mice carrying Tg(EIIa-cre)C5379Lmgd (see Stock No. 003724), Cre recombinase expression in the early mouse embryo results in a decreased electroconvulsive threshold (ECT), some homozygote postnatal lethality, and limbic and tonic clonic grand Mal-like seizures in both homozygotes and heterozygotes. The phenotype is more severe on the C57BL/6 background as compared to the 129S1/SvImJ background.
When bred to mice carrying Tg(CAG-(cre/Esr1*)5Amc (Stock No. 004682), tamoxifen-induced Cre-mediated recombination at 7 weeks of age results a decreased ECT and induced as well as spontaneous seizures.
When bred to mice carrying Emx1tm1(cre)Krj (see Stock No. 005628), Cre recombinase expression in neocortical and hippocampal neurons
results in a decreased ECT and handling associated seizures.
When bred to mice carrying Tg(Slc32a1-cre)2.1Hzo (see Stock No. 017535), Cre recombinase expression in in GABAergic neurons
results in no significant change in ECT and does not result in a seizure phenotype.
A targeting vector was designed to insert a loxP site 2.1 kb upstream of exon 1 followed by a second loxP site and a frt-flanked neomycin resistance (neo) cassette 444 bp downstream of exon 1. Correctly targeted 129-derived R1 ES cells were injected into C57BL/6J blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice to delete the neo cassette. These mice were backcrossed to C57BL/6J for 10 generations.
Allele Name | targeted mutation 1.1, Wayne N Frankel |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Celf4flox |
Gene Symbol and Name | Celf4, CUGBP, Elav-like family member 4 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 18 |
Molecular Note | LoxP sites were inserted 2.1 kb upstream and 444bp downstream of exon 1. An frt-flanked neo selection cassette was deleted from intron 1 in the final allele. |
When using the B6.129-Celf4tm1.1Frk/Frk mouse strain in a publication, please cite the originating article(s) and include JAX stock #018126 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Celf4<tm1Frk> |
Frozen Mouse Embryo | B6.129-Celf4<tm1.1Frk>/Frk Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Celf4<tm1.1Frk>/Frk Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Celf4<tm1.1Frk>/Frk Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Celf4<tm1.1Frk>/Frk Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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