Plcb2 (phospholipase C, beta 2) knockout mice show enhanced resistance to viral and bacterial infection, and lack sweet and bitter taste reception.
Melvin Simon, University of California, San Diego
Plcb2 (phospholipase C, beta 2) hydryolyzes phosphatidylinositol 4,5-bisphosphate to produce two second messengers, inositol triphosphates and diacylglycerol. Expressed in hematopoietic cells and taste tissues, it is involved with chemoattractant-mediated signal transduction.
Knockout mice do not show any defects in hematopoiesis, but chemoattractant-induced Ca2+ release and superoxide production in neutrophils is blocked. Chemotaxis of different leukocyte populations is enhanced. Immune responses to bacteria and viruses are not impaired and may even be enhanced.
Homozygotes lack sweet, amino acid, and bitter taste reception, but sour and salty taste perception are not affected.
These mice may still be segregating for the alleles present in the embryonic stem cells, including coat color alleles.
A portion of two exons encoding residues 378-464 (located in the C terminus of the Xbox) were replaced with a neomycin resistance gene. The mutation was created in 129S1/Sv-p+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. The donating laboratory reports mice were backcrossed four times to C57BL/6 (see SNP results below) prior to sending agouti males to The Jackson Laboratory Repository in 2012. Upon arrival, mutant males were bred to C57BL/6J females (Stock No. 000664), resulting in four mutant offspring (two females and two males). The two males (one agouti and one black) were used to cryopreserve sperm. The two females (both agouti) were bred to C57BL/6J males to establish the living mouse colony. Thereafter, mutant females were bred with mutant males to maintain the living colony. These mice may still be segregating for the alleles present in the embryonic stem cells, including coat color alleles.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation female mice at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be heterozygous. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Dianqing Wu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Plcb2, phospholipase C, beta 2|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Sequence encoding the X box was disrupted by the insertion of a neomycin selection cassette. Western blot analysis of spleen and thymus extracts showed an absence of protein.|
|Mutations Made By|| |
Dianqing Wu, Yale University School of Medicine
Heterozygotes and homozygotes are viable and fertile.
When using the PLC beta2 null mouse strain in a publication, please cite the originating article(s) and include JAX stock #018064 in your Materials and Methods section.