These mice possess loxP sites flanking the promoter and exon 1 of the ferritin heavy chain 1 (Fth1) gene and have applications in studies related to iron homeostasis and oxidative stress.
Lukas C. Kuhn, Ecole polytechnique Federale de Lausanne
These mice possess loxP sites flanking the promoter and exon 1 of the Fth1 (ferritin heavy chain 1) gene. Fth1 is part of the ferritin protein complex and is involved in iron storage and iron homeostasis.
Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the promoter and exon 1 deleted in the cre-expressing tissues.
When bred to a strain with IFN or pI-pC inducible Cre recombinase expression in liver cells and lymphocytes (see Stock No. 003556 for example), this mutant mouse strain may be useful in studies of iron homeostasis.
A loxP site flanked targeting vector containing a FRT site flanked NEO cassette was utilized in the construction of this mutant. A loxP site and the FRT site flanked selection cassette were inserted upstream of the promoter and exon 1 of the targeted gene, and another loxP site was inserted downstream of exon 1. This construct was electroporated into unspecified 129/Sv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to mutant mice expressing FLPe recombinase under the control of the human beta-actin promoter. Mice that retained the loxP site flanked promoter and exon 1 were then bred to C57BL/6 mice to remove the FLPe recombinase allele. The mice were backcrossed to C57BL/6J for 7 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Lukas C Kuhn|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Fth1, ferritin heavy polypeptide 1|
|Strain of Origin||129/Sv|
|Molecular Note||Flp-mediated recombination removed the neo cassette leaving the promoter and exon 1 floxed.|
|Mutations Made By|| |
Lukas Kuhn, Ecole polytechnique Federale de Lausanne
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Fth1tm1.1Lck/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018063 in your Materials and Methods section.