B6.Cg-Cd101^tm1.1Dil/J

Stock number: 018062
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These mice carry a targeted mutation of the Cd101, CD101 antigen, gene. Homozygotes exhibit reduced granulocyte numbers and develop more severe experimentally induced autoimmune primary biliary cirrhosis when compared to wildtype controls.

Detailed description

The transmembrane CD101, also known as IGSF2 (Immunoglobulin superfamily, member 2) is expressed by regulatory T cells (Tregs), effector T cells, granulocytes, dendritic cells, and monocytes and is implicated in T cell proliferation inhibition. These mice carry a targeted mutation of the Cd101, CD101 antigen, gene. Homozygotes are viable, fertile and exhibit a lower granulocyte (Gr1+ cells in the bone marrow) number when compared to wildtype controls. No gene product (protein) is detected by FACS analysis of bone marrow and spleen cells. When challenged with Novosphingobium aromaticivorans exposure, homozygotes develop more severe liver disease than wildtype controls.

Development

A FRT site flanked Neo selection cassette followed by a loxP site and 3 stop codons was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice (on the C57BL/6 genetic background) expressing Cre recombinase(Oz-Cre deleter) to remove the exon 1 and the selection cassette. The mice were backcrossed to C57BL/6NTac for 7 generations using a marker assisted protocol. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Cd101^tm1.1Dil
Name: targeted mutation 1.1, Linda Wicker
MGI accession ID: MGI:5288503
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd101
Marker name: CD101 antigen
Chromosome: 3
Strain of origin: B6.Cg-Thy1^a
Molecular note: A loxP site was inserted upstream of exon 1. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. The absence of protein expression was confirmed by cell sorting on bone marrow and spleen cells.