These mice carry a targeted mutation of the Cd101, CD101 antigen, gene. Homozygotes exhibit reduced granulocyte numbers and develop more severe experimentally induced autoimmune primary biliary cirrhosis when compared to wildtype controls.
Linda Wicker, University of Cambridge, UK
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cd101 | CD101 antigen |
The transmembrane CD101, also known as IGSF2 (Immunoglobulin superfamily, member 2) is expressed by regulatory T cells (Tregs), effector T cells, granulocytes, dendritic cells, and
monocytes and is implicated in T cell proliferation inhibition. These mice carry a targeted mutation of the Cd101, CD101 antigen, gene. Homozygotes are viable, fertile and exhibit a lower granulocyte (Gr1+ cells in the bone marrow) number when compared to wildtype controls. No gene product (protein) is detected by FACS analysis of bone marrow and spleen cells. When challenged with Novosphingobium aromaticivorans exposure, homozygotes develop more severe liver disease than wildtype controls.
A FRT site flanked Neo selection cassette followed by a loxP site and 3 stop codons was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice (on the C57BL/6 genetic background) expressing Cre recombinase(Oz-Cre deleter) to remove the exon 1 and the selection cassette. The mice were backcrossed to C57BL/6NTac for 7 generations using a marker assisted protocol. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Linda Wicker |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Cd101, CD101 antigen |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 3 |
Molecular Note | A loxP site was inserted upstream of exon 1. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. The absence of protein expression was confirmed by cell sorting on bone marrow and spleen cells. |
Mutations Made By | Linda Wicker, University of Cambridge, UK |
When maintaining a live colony, these mice are bred as homozygotes.
When using the B6(Cg)-Cd101tm1.1Dil/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018062 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd101<tm1.1Dil> |
Frozen Mouse Embryo | B6(Cg)-Cd101<tm1.1Dil>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Cd101<tm1.1Dil>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Cd101<tm1.1Dil>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Cd101<tm1.1Dil>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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