B6;129S5-Glmn^{Gt(OST109778)Lex}/JdecJ

Stock number: 018060
Research areas: Developmental Biology Research, Research Tools

Description

This gene trap mutation abolishes endogenous Glmn, glomulin, FKBP associated protein, gene function. These mice may be useful for studying the ubiquitin-proteasome pathway, and early embryonic growth and development.

Detailed description

Glomulin, FKBP associated protein, interacts with cullin 7, and is involved in the post-translational protein modification processes ubiquitination and neddylation. These mice carry a knockout mutation of the Glmn gene. Mice that are heterozygous for the mutation are viable and fertile. Homozygous embryos die between E10.5 and E12.5. No gene product (mRNA) is detected in homozygous E9.5 embryos by RT-qPCR. Homozygous embryos, aged E9.5, exhibit delayed growth and neural tube closure, incomplete axial turning, pericardial effusion, pooling of blood, and disorganized yolk sac vascular network.

Development

A "gene trap" targeting vector was used replace the endogenous glomulin, FKBP associated protein (Glmn) gene with a neomycin (neo) resistance cassette followed by the simian virus 40 polyadenylylation signal. This gene trap targeting vector integrated into intron 1 via electroporation into 129S5/SvEvBrd-derived Lex-1 embryonic stem (ES) cells. These OST109778 ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred with C57BL/6J mice. Next, sperm from heterozygous mice was provided to The Jackson Laboratory Repository. This sperm was used to fertilize C57BL/6J oocytes, and the resulting mice were used to establish the colony.

Allele

Symbol: Glmn^{Gt(OST109778)Lex}
Name: gene trap OST109778, Lexicon Genetics
MGI accession ID: MGI:3817643
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Marker symbol: Glmn
Marker name: glomulin, FKBP associated protein
Chromosome: 5
Strain of origin: 129S5/SvEvBrd
Molecular note: The Lexicon Genetics gene trap vector VICTR48, which contains a splice acceptor sequence followed by the neomycin resistance gene and a double polyadenylation signal, has integrated into the first intron of the glomulin gene upstream of the translation initiation site, which resides in the second exon (Genbank accession NM_133248.1). Western blot analysis confirmed the absence of protein expression in the yolk sac.