This gene trap mutation abolishes endogenous Glmn, glomulin, FKBP associated protein, gene function. These mice may be useful for studying the ubiquitin-proteasome pathway, and early embryonic growth and development.
James A. DeCaprio, Dana Farber Cancer Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Glmn | glomulin, FKBP associated protein |
Glomulin, FKBP associated protein, interacts with cullin 7, and is involved in the post-translational protein modification processes ubiquitination and neddylation. These mice carry a knockout mutation of the Glmn gene. Mice that are heterozygous for the mutation are viable and fertile. Homozygous embryos die between E10.5 and E12.5. No gene product (mRNA) is detected in homozygous E9.5 embryos by RT-qPCR. Homozygous embryos, aged E9.5, exhibit delayed growth and neural tube closure,
incomplete axial turning, pericardial effusion, pooling of blood, and disorganized yolk sac vascular network.
A "gene trap" targeting vector was used replace the endogenous glomulin, FKBP associated protein (Glmn) gene with a neomycin (neo) resistance cassette followed by the simian virus 40 polyadenylylation signal. This gene trap targeting vector integrated into intron 1 via electroporation into 129S5/SvEvBrd-derived Lex-1 embryonic stem (ES) cells. These OST109778 ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred with C57BL/6J mice. Next, sperm from heterozygous mice was provided to The Jackson Laboratory Repository. This sperm was used to fertilize C57BL/6J oocytes, and the resulting mice were used to establish the colony.
Allele Name | gene trap OST109778, Lexicon Genetics |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Glmn, glomulin, FKBP associated protein |
Gene Synonym(s) | |
Strain of Origin | 129S5/SvEvBrd |
Chromosome | 5 |
Molecular Note | The Lexicon Genetics gene trap vector VICTR48, which contains a splice acceptor sequence followed by the neomycin resistance gene and a double polyadenylation signal, has integrated into the first intron of the glomulin gene upstream of the translation initiation site, which resides in the second exon (Genbank accession NM_133248.1). Western blot analysis confirmed the absence of protein expression in the yolk sac. |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype.
When using the B6;129S5-GlmnGt(OST109778)Lex/JdecJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #018060 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Glmn<Gt(OST109778)Lex> |
Frozen Mouse Embryo | B6;129S5-Glmn<Gt(OST109778)Lex>/JdecJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S5-Glmn<Gt(OST109778)Lex>/JdecJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S5-Glmn<Gt(OST109778)Lex>/JdecJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S5-Glmn<Gt(OST109778)Lex>/JdecJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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