These targeted mutation mice carry a functional knockout of the Oscar gene, disrupting a pathways associated with osteoclastogenesis. These mice may be useful in studies of bone remodeling and the pathological bone loss observed in autoimmune diseases, such as osteoporosis and rheumatoid arthritis, and bone cancers.
Marco Colonna, Washington University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Oscar | osteoclast associated receptor |
Oscar (osteoclast associated receptor) is a collagen receptor, specifically expressed by pre-osteoclasts, that acts as a costimulatory signal for the Fc receptor common gamma (FcRg)-associated pathway required for osteoclastogenesis.
To better define the role of Oscar in osteoclast differentiation, knockout mice were generated. Animals homozygous for a Oscar knockout do not exhibit any difference in bone volume when compared with wildtype mice.
When the Oscar knockout is combined with a Tyrobp (Dap12) knockout, animals show decreased osteoclast numbers, decreased osteoclast size, and a reduction in eroded bone surfaces as compared to animals homozygous for a Tyrobp targeted mutation. The number and volume of trabeculae in bones is increased. Osteoblast numbers and bone formation parameters are unchanged.
These mice may be useful in studies of bone remodeling and the pathological bone loss observed in autoimmune diseases, such as osteoporosis and rheumatoid arthritis, and bone cancers.
An approximately 3 kb region encoding the entire extracellular domain (exons 3 and 4) and transmembrane domain (exon 5) was deleted from the gene and replaced with a neomycin drug resistance cassette. The mutation was created in E14.1 129P2/OlaHsd-derived embryonic stem (ES) cells. Animals were backcrossed to C57BL/6J to create speed congenics with a greater than 99% C57BL/6 background.
Allele Name | targeted mutation 1, Yongwon Choi |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Oscar, osteoclast associated receptor |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | A 3.0 kb segment, containing the entire extracellular domain (exon 3 and 4) and transmembrane domain (exon 5), was deleted. |
Animals homozygous for both mutations are viable and fertile.
When using the B6.129P2-Oscartm1Ywc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018053 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Oscar<tm1Ywc> |
Frozen Mouse Embryo | B6.129P2-Oscar<tm1Ywc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Oscar<tm1Ywc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Oscar<tm1Ywc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Oscar<tm1Ywc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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