The Fkbp5 gene is disrupted by lacZ in this knockout strain. Aged mice exhibit anti-depressive behavior in behavioral tests, reduced stress corticosterone levels and may be useful for studying anxiety and depressive disorders, as well as endothelial barrier integrity.
Chad Dickey, USF Health Alzheimer's Institute
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Fkbp5 | FK506 binding protein 5 |
FKBP5 (or FKBP51) functions as a co-chaperone that binds to HSP90 and is involved in regulation of the glucocorticoid receptor. Mutations that increase protein levels of human FKBP5 are associated with depressive disorders, anxiety, bipolar disorders, and post-traumatic stress syndrome. In this strain, the targeting vector includes a lacZ gene and replaces the Fkbp5 first coding exon. Expression of beta-galactosidase is under the control of the endogenous promoter. Expression in the brain increases with age particularly in the upper cortical layers of the forebrain. Homozygous mice are viable and fertile. Aged mice (over a year old) exhibit shorter immobility time in forced swim and tail suspension tests, and reduced stress corticosterone levels. This strain may be useful for studying anxiety and depressive disorders.
Fkbp5 knock-out mice have also been used to demonstrate that FKBP51 is a novel regulator of endothelial barrier integrity. This finding is significant as it reveals a protective mechanism for endothelium against calcium entry-induced disruption.
A targeting vector containing the lacZ gene and a PGKneo cassette was used to replace exon 2, the first coding exon. Expression of beta-galactosidase is under the control of the endogenous promoter and is expressed in frame with the initiation codon. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred C57BL/6 mice. The strain was maintained at the Mayo Clinic and then transferred to the laboratory of Dr. Chad Dickey at the University of South Florida. The Fkbp5 allele was maintained on a mixed Swiss-Webster and C57BL/6 background for many generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | lacZ is expressed in areas of the brain. |
Allele Name | targeted mutation 1, David F Smith |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | 51KO; Fkbp51- |
Gene Symbol and Name | Fkbp5, FK506 binding protein 5 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in areas of the brain. |
Strain of Origin | 129X1/SvJ |
Chromosome | 17 |
Molecular Note | Exon 2 was replaced with a lacZ and neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. |
While maintaining a live colony, these mice can be bred as homozygotes. The Donating Investigator uses a heterozygote x heterozygote breeding strategy on an outbred background to maintain breeding performance.
When using the FKbp51 mouse strain in a publication, please cite the originating article(s) and include JAX stock #017989 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Fkbp5<tm1Dvds> |
Frozen Mouse Embryo | STOCK Fkbp5<tm1Dvds>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Fkbp5<tm1Dvds>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Fkbp5<tm1Dvds>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Fkbp5<tm1Dvds>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.