These mice express cre recombinase under the direction of Hoxb6 and are useful for studying gene function during the early stages of limb development.
Michael Kuehn, NIH\NCI Frederick
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These mice express Cre recombinase under the control of the mouse homeobox B6 (Hoxb6) lateral plate mesoderm (LPM) enhancer region. HOXB6 is a transcription factor that regulates anterior-posterior axis positioning during development. Hoxb6-Cre is expressed throughout the lateral plate mesoderm at E8.5, in mesoderm of the hindlimb bud at E9.5, and in the entire hindlimb by E11.5. In the forelimb, Cre activity is detected at the posterior end during limb initiation and then extends anterior until nearly the entire limb bud is positive by E11.5. Homozygotes are viable and fertile. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in Cre-expressing tissues of the offspring.
For example, when bred to a strain expressing a floxed-fibroblast growth factor receptor 1 (Fgfr1) gene, the resulting mice exhibit malformed hindlimbs and abnormal posterior portions of the forelimbs. The limb buds display reduced proximal-distal height and increased dorsal-ventral length.
A transgenic construct containing cre coding sequence under the control of the mouse homeobox B6 (Hoxb6) lateral plate mesoderm (LPM) enhancer region was introduced into FVB/N donor oocytes. The resulting Hoxb6-Cre offspring were bred to FVB/N mice to establish a colony. These mice were then bred to mice on unknown backgrounds, and the donating investigator reports that the mice had all other contaminating alleles bred out of the mice sent to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | This promoter is active in extraembryonic mesoderm at E7.5, the lateral plate mesoderm and the midbrain/hindbrain boundary by E8.5-E9.5, and the developing fore and hind limbs by E10.5-E11.5. |
Allele Name | transgene insertion, Michael R Kuehn |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | HoxB6 Cre; Hoxb6::Cre; HoxB6-Cre; Tg(Hoxb6-cre)Mku |
Gene Symbol and Name | Tg(Hoxb6-cre)#Mku, transgene insertion, Michael R Kuehn |
Gene Synonym(s) | |
Promoter | Hoxb6, homeobox B6, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | This promoter is active in extraembryonic mesoderm at E7.5, the lateral plate mesoderm and the midbrain/hindbrain boundary by E8.5-E9.5, and the developing fore and hind limbs by E10.5-E11.5. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of the Hoxb6 promoter. This promoter is active in extraembryonic mesoderm at E7.5, the lateral plate mesoderm and the midbrain/hindbrain boundary by E8.5-E9.5, and the developing fore and hind limbs by E10.5-E11.5. |
Mutations Made By | Michael Kuehn, NIH\NCI Frederick |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Tg(Hoxb6-cre)#Mku/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017981 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Hoxb6-cre)Mku |
Frozen Mouse Embryo | STOCK Tg(Hoxb6-cre)#Mku/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Hoxb6-cre)#Mku/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Hoxb6-cre)#Mku/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Hoxb6-cre)#Mku/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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