These Vglut2-ChR2-YFP transgenic mice express channelrhodopsin-2/EYFP fusion protein directed to locomotor regions of the spinal chord and hindbrain. This strain provides a useful tool for inducible photostimulation of glutamatergic neurons involved in rhythm generation and initiation of locomotion.
Ole Kiehn, Karolinska Institutet,
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for the Vglut2-ChR2-YFP transgene (or Tg(Slc17a6-COP4 *H134R/EYFP)2Oki) are viable and fertile, with expression of the ChR2-YFP fusion protein directed to spinal cord and hindbrain by the mouse glutamate transporter Slc17a6 promoter region on the BAC transgene. Slc17a6 is found in neurons of the locomotor region of the spinal cord and is the dominant transporter found in the hindbrain. Under blue light, ChR2 activates YFP-expressing cells and induces neuronal activity of the glutamatergic population in spinal and hindbrain locomotor regions. This strain provides a useful tool for inducible photostimulation of glutamatergic neurons involved in rhythm generation and initiation of locomotion.
A bacterial articificial chromosone (BAC) # RPCI23-84M15 containing the mouse Vglut2 gene was modified by the introduction of a human codon-optimized channel rhodopsin (hChR2) and a gain-of-function H134R substitution fused to an enhanced yellow fluorescent protein sequence (EYFP) and inserted to inactivate the Vglut2 gene. The BAC vector backbone was removed and microinjected into the pronuclei of oocytes from C57BL/6N females. The donating investigator reported that founder line 2 was established and bred to C57BL/6N mice to generate the colony (see SNP note below). The strain has been maintained as a cross to C57BL/6NJ inbred mice (Stock No. 005304).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J;C57BL/6N genetic background.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas |
Site of Expression | These transgenic mice express channelrhodopsin-2/EYFP fusion protein directed to locomotor regions of the spinal chord and hindbrain. |
Allele Name | transgene insertion 2, Ole Kiehn |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Vglut2-ChR2-YFP; vGluT2-YFP |
Gene Symbol and Name | Tg(Slc17a6-COP4*H134R/EYFP)2Oki, transgene insertion 2, Ole Kiehn |
Gene Synonym(s) | |
Promoter | Slc17a6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas |
Site of Expression | These transgenic mice express channelrhodopsin-2/EYFP fusion protein directed to locomotor regions of the spinal chord and hindbrain. |
Strain of Origin | C57BL/6N |
Chromosome | UN |
Molecular Note | A bacterial articificial chromosone (BAC) # RPCI23-84M15 containing the mouse Vglut2 gene was modified by the introduction of a human codon-optimized channel rhodopsin (hChR2) and a gain-of-function H134R substitution fused to an enhanced yellow fluorescent protein sequence (EYFP) and inserted to inactivate the Vglut2 gene. Line 2 was generated. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Vglut2-ChR2-YFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #017978 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Slc17a6-COP4*H134R/EYFP)2Ok |
Frozen Mouse Embryo | C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J Frozen Embryo | $3373.50 |
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