These transgenic mice allow inducible astroglia-specific expression of human MAOB (monoamine oxidase B) and lacZ (beta-galactosidase). With induction, they exhibit neurodegeneration, decreased locomotor activity, mitochondrial dysfunction, astrogliosis, and microglial activation. This strain may have applications in studies related to the initiation and progression of Parkinson's disease.
Julie K. Andersen, Buck Institute for Age Research
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence, Transactivator) |
Monoamine oxidase B catalyzes dopamine oxidative de-amination predominantly in glial cells, producing large amounts of reactive oxygen species. Monoamine oxidase B inhibitors act to increase synaptic dopamine by blocking degradation and are used in treatment to delay the progression of symptoms of Parkinson's disease.
These transgenic mice have inducible astroglia-specific expression of human MAOB (monoamine oxidase B) and lacZ (beta-galactosidase) under the control of the tetO, tetracycline-responsive regulatory element. Administration of doxycycline induces an approximately 2.5 fold increase in astrocyte-specific expression of MAOB. Transgene expression is detected only after induction in the brain by RT-PCR and specifically in astrocytes by immunohistochemical analysis. Induced elevated levels of MAOB result in progressive loss of dopaminergic neurons in the substantia nigra, a decrease in mitochondrial complex I activity and an increase in mitochondrial oxidative stress in dopaminergic neurons in the substantia nigra. Doxycycline treated transgenic mice exhibit astrogliosis, decreased locomotor activity and nigrostriatal microglial activation. The Donating Investigator reports that mice that are homozygous for the transgene are viable, and normal in size.
A transgenic construct containing a human MAOB (monoamine oxidase B) cDNA and lacZ (beta-galactosidase) gene under the control of the tetO, tetracycline-responsive regulatory element, and a second transgenic construct containing rtTA (reverse tetracycline-controlled transactivator) under the control of the mouse Gfap (glial fibrillary acidic protein) promoter were co-injected into fertilized C57BL/6 mouse eggs. Founder animals were bred to C57BL/6, and maintained on the C57BL/6 background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | MAOB, monoamine oxidase B, human |
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Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression |
Allele Name | transgene insertion 1, Julie K Andersen |
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Allele Type | Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence, Transactivator) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan, transgene insertion 1, Julie K Andersen |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Promoter | Gfap, glial fibrillary acidic protein, mouse, laboratory |
Expressed Gene | MAOB, monoamine oxidase B, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | This transgene consists of 2 co-injected constructs. One encodes for a reverse tetracycline responsive transactivator with expression driven by a murine Gfap promoter. The second has a bidirectional tetracycline responsive promoter that drives expression of a lacZ reporter and a human MOAB cDNA. Several founders were generated and the line with the highest level of expression was used for analyses. Line 1 was generated. |
Mutations Made By | Julie Andersen, Buck Institute for Age Research |
When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes.
When using the C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017955 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan |
Frozen Mouse Embryo | C57BL/6-Tg(Gfap-rtTAtetO-MAOB-lacZ)1Jkan/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Gfap-rtTAtetO-MAOB-lacZ)1Jkan/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Gfap-rtTAtetO-MAOB-lacZ)1Jkan/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Gfap-rtTAtetO-MAOB-lacZ)1Jkan/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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