These transgenic mice express a FLAG-tagged Gfra1 under the control of a Mnx1 (motor neuron and pancreas homeobox 1; also called Hb9) motor neuron-specific promoter fragment. Mice show defects in hindlimb innervation.
Samuel L Pfaff, The Salk Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Gfra1 (glial cell line derived neurotrophic factor family receptor alpha 1) is expressed by limb-innervating motor neurons. These transgenic mice express a FLAG-tagged Gfra1 under the control of a Mnx1 (motor neuron and pancreas homeobox 1; also called Hb9) motor neuron-specific promoter fragment.
These mice show defects in hindlimb innervation. Significant thinning of the peroneal nerve indicates a reduced contribution of LMCL (lateral division of the lateral motor column) axons to the dorsal branch. Additionally, these LMCL axons misproject into the ventral hindlimb of embryos. Other motor neuron pathways including the ventral branch of the hindlimb and dorsal ramus directed to axial musculature are unaffected in embryos at E10.5-13.5. Innervation of the cutaneous maximus and latissimus dorsi muscles by brachial motor neurons that depend on GDNF (glial cell line derived neurotrophic factor) is normal.
A 9.2 kb region of the mouse Mnx1 (Hb9) promotor drives expression of the mouse Gfra1 cDNA containing a FLAG-tag insert between the signal sequence and the start codon. The construct utilizes an SV40 poly-A sequence. This mutation was introduced to CB6F1 hybrid embryos and maintained on a mixed BALB/c - C57BL/6 genetic background by the donating laboratory.
Expressed Gene | Gfra1, glial cell line derived neurotrophic factor family receptor alpha 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Samuel L Pfaff |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Hb9::Gfra1 |
Gene Symbol and Name | Tg(Mnx1-Gfra1)1Slp, transgene insertion 1, Samuel L Pfaff |
Gene Synonym(s) | |
Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory |
Expressed Gene | Gfra1, glial cell line derived neurotrophic factor family receptor alpha 1, mouse, laboratory |
Strain of Origin | (BALB/c x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | A 9.2 kb region of the mouse Mnx1 (Hb9) promotor drives expression of the mouse cDNAcontaining a FLAG-tag insert between the signal sequence and the start codon. The construct utilizes an SV40 poly-A sequence. Line 1 was generated. |
Mutations Made By | Samuel Pfaff, The Salk Institute |
Heterozygotes or homozygotes may be bred.
When using the B6;C-Tg(Mnx1-Gfra1)1Slp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017953 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Mnx1-Gfra1)1Slp |
Frozen Mouse Embryo | B6;C-Tg(Mnx1-Gfra1)1Slp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C-Tg(Mnx1-Gfra1)1Slp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C-Tg(Mnx1-Gfra1)1Slp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;C-Tg(Mnx1-Gfra1)1Slp/J Frozen Embryo | $3373.50 |
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