Grlf1 (glucocorticoid receptor DNA binding factor 1, also called p190 RhoGAP (GTPase activating protein)) is a potent Rho regulator that mediates integrin-dependent adhesion signaling in cultured cells. The gene is expressed at high levels throughout the developing nervous system. 
This ENU-generated I602N mutation of Grlf1 creates a severe hypomorphic allele that displays phenotypes equivalent to those reported for the loss-of-function allele described in Brouns MR et al., Development 2000 Nov;127(22):4891-903. A small percentage of embryos are exencephalic and show neural tube closure defects that are likely linked to misregulation of cell adhesion processes. 

Levels of the I602N transcript are unchanged in spinal cord and brain tissues, but protein levels are reduced. Mice homozygous for the mutation usually die within 2 days of birth.

This ENU-generated mutation of Grlf1 creates a severe hypopmorphic allele associated defective cell adhesion signaling and abnormal development of the nervous system.

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