These transgenic mice contain the myelin protein zero (P0) (Mpz) promoter driving expression of Cre recombinase in Schwann cells. These mice may be useful for studying the myelination of peripheral nerve cells.
Dr. M Laura Feltri, University of Buffalo
Dr. Lawrence Wrabetz, University of Buffalo
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These mice express Cre recombinase under the control of the myelin protein zero (P0) (Mpz) gene. P0 is a glycoprotein located in Schwann cells of the peripheral nervous system (but not in sensory neurons). Defects in Schwann cells lead to nerve cell demyelination and have been associated with Charcot-Marie-Tooth disease and Dejerine-Sottas disease. Cre-recombinase expression is seen primarily in Schwann cells, and to a lesser extent in a small number of periventricular cells that give rise to cells in the cortex. Expression is also seen in a subpopulation of neural-crest derived progenitors in the heart. Homozygotes are viable and fertile. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in Schwann cells between embryonic days 13.5 and 14.5.
For example, when bred to a strain expressing a floxed-Beta1 integrin (Bint1) gene, the resulting mice exhibit tremor, abnormal gait, paw clenching and defects in radial sorting of axons by Schwann cells and altered myelination. Analysis of BINT1 expression shows that recombination occurs in Schwann cells, but not in dorsal root ganglion neurons (Feltri et al., J Cell Biol. 2002).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing cre coding sequence under the control of the myelin protein zero (Mpz) promoter was introduced into FVB/NTac donor oocytes where multiple copies of it integrated into the 35-45 cM position on Chromosome 15. The resulting mPoTOTA-Cre offspring were bred to FVB/NTac mice and subsequently to C57BL/6NCrl mice for at least 15 generations to develop this congenic line. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ mice (Stock No. 005304) to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in Schwann cells, but not in sensory neurons. |
Allele Name | transgene insertion 26, Albee Messing |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | mP0TOTA(Cre); mPoTOTA-Cre; MPZ(Cre); P0-Cre; Tg(Mpz-cre)1Mfel; Tg98.1 |
Gene Symbol and Name | Tg(Mpz-cre)26Mes, transgene insertion 26, Albee Messing |
Gene Synonym(s) | |
Promoter | Mpz, myelin protein zero, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in Schwann cells, but not in sensory neurons. |
Strain of Origin | FVB/NTac |
Chromosome | 15 |
Molecular Note | This transgene expresses Cre recombinase under the control of a mouse Mpz promoter. This transgene is expressed in Schwann cells, but not in sensory neurons. Multiple copies of the transgene construct were integrated into the 35-45 cM position on Chromosome 15. |
Mutations Made By | Dr. Albee Messing, University of Wisconsin-Madison |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304).
When using the P0-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017927 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Mpz-cre)26Mes |
Frozen Mouse Embryo | B6N.FVB-Tg(Mpz-cre)26Mes/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB-Tg(Mpz-cre)26Mes/J | $2595.00 |
Frozen Mouse Embryo | B6N.FVB-Tg(Mpz-cre)26Mes/J | $3373.50 |
Frozen Mouse Embryo | B6N.FVB-Tg(Mpz-cre)26Mes/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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